Differential transcript profile of inhibitors with potential anti-venom role in the liver of juvenile and adult Bothrops jararaca snake

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dc.citation.volume5
dc.contributor.authorGomes, Cicera Maria
dc.contributor.authorde Morais-Zani, Karen [UNIFESP]
dc.contributor.authorLu, Stephen [UNIFESP]
dc.contributor.authorBuarque, Diego de Souza [UNIFESP]
dc.contributor.authorCardoso Braz, Gloria Regina
dc.contributor.authorGrego, Kathleen Fernandes
dc.contributor.authorTanaka, Aparecida Sadae [UNIFESP]
dc.contributor.authorTanaka-Azevedo, Anita Mitico
dc.coverageLondon
dc.date.accessioned2020-07-17T14:02:15Z
dc.date.available2020-07-17T14:02:15Z
dc.date.issued2017
dc.description.abstractBackground. Snakes belonging to the Bothrops genus are vastly distributed in Central and South America and are responsible for most cases of reported snake bites in Latin America. The clinical manifestations of the envenomation caused by this genus are due to three major activities-proteolytic, hemorrhagic and coagulant-mediated by metalloproteinases, serine proteinases, phospholipases A(2) and other toxic compounds present in snake venom. Interestingly, it was observed that snakes are resistant to the toxic effects of its own and other snake's venoms. This natural immunity may occur due the absence of toxin target or the presence of molecules in the snake plasma able to neutralize such toxins. Methods. In order to identify anti-venom molecules, we construct a cDNA library from the liver of B. jararaca snakes. Moreover, we analyzed the expression profile of four molecules-the already known anti-hemorrhagic factor Bj46a, one gamma-phospholipase A(2) inhibitor, one inter-alpha inhibitor and one C1 plasma protease inhibitor-in the liver of juvenile and adult snakes by qPCR. Results. The results revealed a 30-fold increase of gamma-phospholipase A(2) inhibitor and a minor increase of the inter-alpha inhibitor (5-fold) and of the C1 inhibitor (3-fold) in adults. However, the Bj46a factor seems to be equally transcribed in adults and juveniles. Discussion. The results suggest the up-regulation of different inhibitors observed in the adult snakes might be a physiological adaptation to the recurrent contact with their own and even other snake's venoms throughout its lifespan. This is the first comparative analysis of ontogenetic variation of expression profiles of plasmatic proteins with potential anti-venom activities of the venomous snake B. jararaca. Furthermore, the present data contributes to the understanding of the natural resistance described in these snakes.en
dc.description.affiliationInst Butantan, Lab Herpetol, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Interunidades Biotecnol, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Bioquim, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Dept Bioquim, Rio De Janeiro, Brazil
dc.description.affiliationInst Nacl Ciencia & Tecnol Entomol Mol, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Bioquim, Escola Paulista Med, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Bioquim, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Tecnologico (CNPq)
dc.description.sponsorshipINCT - Entomologia Molecular
dc.description.sponsorshipIDFAPESP: 2010/10266-5
dc.description.sponsorshipIDFAPESP: 2012/03657-8
dc.description.sponsorshipIDFAPESP: 2013/05357-4
dc.description.sponsorshipIDFAPESP: 2014/11108-0
dc.description.sponsorshipIDCNPq: 308780/2013-2
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.7717/peerj.3203
dc.identifier.citationPeerj. London, v. 5, p. -, 2017.
dc.identifier.doi10.7717/peerj.3203
dc.identifier.fileWOS000400305500003.pdf
dc.identifier.issn2167-8359
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54707
dc.identifier.wosWOS:000400305500003
dc.language.isoeng
dc.publisherPeerj Inc
dc.relation.ispartofPeerj
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectProtease inhibitorsen
dc.subjectAnti-venomen
dc.subjectSnake plasmaen
dc.subjectGene expressionen
dc.subjectPlasmatic proteinen
dc.subjectBothrops jararacaen
dc.titleDifferential transcript profile of inhibitors with potential anti-venom role in the liver of juvenile and adult Bothrops jararaca snakeen
dc.typeinfo:eu-repo/semantics/article
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