Diversity of mechanisms conferring resistance to beta-lactams among OXA-23-producing Acinetobacter baumannii clones

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dc.citation.issue1
dc.citation.volume85
dc.contributor.authorCardoso, Juliana Provasi [UNIFESP]
dc.contributor.authorCayo, Rodrigo [UNIFESP]
dc.contributor.authorGirardello, Raquel [UNIFESP]
dc.contributor.authorGales, Ana Cristina [UNIFESP]
dc.coverageNew York
dc.date.accessioned2020-07-22T13:23:01Z
dc.date.available2020-07-22T13:23:01Z
dc.date.issued2016
dc.description.abstractA total of 31 unrelated OXA-23-producing Acinetobacter baumannii strains isolated from 14 hospitals located in distinct Brazilian regions were evaluated in this study. These isolates were grouped into 12 different sequence types (STs), of which 7 had unique allelic sequences (ST188, ST189, ST190, ST191, ST192, ST228, and ST299). Most isolates belonged to the clonal complex CC79 followed by CC15 and CC1. Only polymyxin B and minocycline showed good activity against the OXA-23-producing A. baumannii clones. The ISAba1 upstream bla(OXA-23), bict(OXA-51)-like, or ampC was found in 100%, 54.8%, and 77.4% of the isolates, respectively. High resistance rates to ceftazidime and cefotaxime were observed among those isolates possessing ISAba1 upstream ampC, in contrast to those isolates that did not carry this configuration. Moreover, a >= 2 Log(2) decrease in the MICs of meropenem and ceftazidime was observed in the presence of phenyl-arginine-beta-naphthylamide for 80.6% and 54.8% of isolates, respectively. Overexpression of the adeB was observed in 61.3% of isolates, particularly among those isolates belonging to the ST1 (CC1). It was also verified that ompW was down -regulated in all isolates belonging to the ST15 (CC15). On the other hand, carO and omp33-36 genes were overexpressed in 48.4% and 58.1% of the isolates, respectively. In this study, we show that overexpression of AdeABC system could significantly contribute for resistance to meropenem and ceftazidime among OXA-23-producing A. baumannii clones in Brazil, demonstrating the complexity involved in the p-lactam resistance in such isolates. (C) 2016 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Med, Lab Alerta, Disciplina Infectol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Med, Lab Alerta, Disciplina Infectol, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipNational Council for Science and Technological Development (CNPq)
dc.description.sponsorshipIDFAPESP: 2012/15459-6
dc.description.sponsorshipIDCNPq: 307816/2009-5
dc.format.extent90-97
dc.identifierhttp://dx.doi.org/10.1016/j.diagmicrobio.2016.01.018
dc.identifier.citationDiagnostic Microbiology And Infectious Disease. New York, v. 85, n. 1, p. 90-97, 2016.
dc.identifier.doi10.1016/j.diagmicrobio.2016.01.018
dc.identifier.issn0732-8893
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55986
dc.identifier.wosWOS:000375358000021
dc.language.isoeng
dc.publisherElsevier Science Inc
dc.relation.ispartofDiagnostic Microbiology And Infectious Disease
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAcinetobacter baumanniien
dc.subjectEfflux pumpsen
dc.subjectPorinsen
dc.subjectAmpCen
dc.subjectOXA-23en
dc.subjectCarbapenemsen
dc.subjectExtended-spectrum cephalosporinsen
dc.subjectMolecular epidemiologyen
dc.titleDiversity of mechanisms conferring resistance to beta-lactams among OXA-23-producing Acinetobacter baumannii clonesen
dc.typeinfo:eu-repo/semantics/article
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