Aldefluor protocol to sort keratinocytes stem cells from skin

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dc.citation.issue11
dc.citation.volume32
dc.contributor.authorRibeiro Noronha, Samuel Marcos [UNIFESP]
dc.contributor.authorGragnani, Alfredo [UNIFESP]
dc.contributor.authorCalado Pereira, Thiago Antonio [UNIFESP]
dc.contributor.authorAlves Correa, Silvana Aparecida [UNIFESP]
dc.contributor.authorBonucci, Jessica [UNIFESP]
dc.contributor.authorFerreira, Lydia Masako [UNIFESP]
dc.coverageSao Paulo
dc.date.accessioned2020-09-01T13:21:21Z
dc.date.available2020-09-01T13:21:21Z
dc.date.issued2017
dc.description.abstractPurpose: To investigate the use Aldefluor r and N, N-Dimethylaminobenzaldehyde (DEAB) to design a protocol to sort keratinocyte stem cells from cultured keratinocytes from burned patients. Methods: Activated Aldefluor r aliquots were prepared and maintained at temperature between 2 to 8 degrees C, or stored at -20 degrees C. Next, the cells were collected following the standard protocol of sample preparation. Results: Best results were obtained with Aldefluor r 1.5 mu l and DEAB 15 mu l for 1 x 10(6) cells, incubated at 37 degrees C for 15 minutes. Flow cytometer range for keratinocyte stem cells separation was evaluated. There were 14.8% of stem cells separated in one sample of keratinocyte culture used to pattern the protocol. After being defined the ideal concentration, the same test pattern was performed in other keratinocyte samples. We observed a final mean of 10.8%. Conclusion: Aldefluor r has been shown as a favorable marking of epidermal keratinocyte stem cells for subsequent separation on a flow cytometer, with detection of 10.8% of epidermal keratinocyte stem cells, in this protocol.en
dc.description.affiliationUniv Fed Sao Paulo, UNIFESP, Div Plast Surg, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Div Plast Surg, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, UNIFESP, Div Plast Surg, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP
dc.description.sponsorshipIDFAPESP: 2013/10905-0
dc.format.extent984-994
dc.identifierhttp://dx.doi.org/10.1590/s0102-865020170110000010
dc.identifier.citationActa Cirurgica Brasileira. Sao Paulo, v. 32, n. 11, p. 984-994, 2017.
dc.identifier.doi10.1590/s0102-865020170110000010
dc.identifier.fileS0102-86502017001100984.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502017001100984
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/58223
dc.identifier.wosWOS:000417846700010
dc.language.isoeng
dc.publisherActa Cirurgica Brasileira
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectKeratinocytesen
dc.subjectFlow Cytometryen
dc.subjectAdult Stem Cellsen
dc.titleAldefluor protocol to sort keratinocytes stem cells from skinen
dc.typeinfo:eu-repo/semantics/article
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