Mouse B-1 cell-derived mononuclear phagocyte, a novel cellular component of acute non-specific inflammatory exudate

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dc.contributor.authorAlmeida, Sandro Rogério de [UNIFESP]
dc.contributor.authorAroeira, L. S.
dc.contributor.authorFrymuller, E. [UNIFESP]
dc.contributor.authorDias, Maria Ângela Amorim [UNIFESP]
dc.contributor.authorBogsan, Cristina Stewart Bittencourt [UNIFESP]
dc.contributor.authorLopes, José Daniel [UNIFESP]
dc.contributor.authorMariano, Mario [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInst Nucl & Energet Res
dc.date.accessioned2016-01-24T12:31:27Z
dc.date.available2016-01-24T12:31:27Z
dc.date.issued2001-09-01
dc.description.abstractAt least three B cell subsets, B-1a, B-1b and B-2, or conventional B cells are present in the mouse periphery. Here we demonstrate that B-1 cells spontaneously proliferate in stationary cultures of normal adherent mouse peritoneal cells. B-1 cells were characterized by morphology, immunohistochemistry and flow cytometry. IgM was detected in the supernatants of these cultures. We demonstrated that the major cell population analyzed expresses the B-1b phenotype. When these cells were transferred to a new culture, a large proportion of them adhere to the plastic surface, and spread as bipolar cells endowed with the capacity to phagocytose via Fe and mannose receptors. Flow cytometry analysis of these adherent cells demonstrated that the great majority of them share both B-220 and Mac-1 antigens. Nevertheless, 45% of them were exclusively Mac-1(+). Finally, when they were labeled in vitro with [H-3]thymidine and transferred to the peritoneal cavity of naive mice, they migrate to a non-specific inflammatory focus induced by a foreign-body implant. These data demonstrate that B-1 cells, mainly B-1b cells, not only proliferate and differentiate into a mononuclear phagocyte in vitro, but also that they exit the peritoneal cavity and migrate to a non-specific inflammatory milieu.en
dc.description.affiliationUniversidade Federal de São Paulo, Discipline Immunol, Dept Microbiol Immunol & Parasitol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Ctr Electron Microscopy, BR-04023900 São Paulo, Brazil
dc.description.affiliationInst Nucl & Energet Res, BR-05508900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Discipline Immunol, Dept Microbiol Immunol & Parasitol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Ctr Electron Microscopy, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1193-1201
dc.identifierhttp://dx.doi.org/10.1093/intimm/13.9.1193
dc.identifier.citationInternational Immunology. Oxford: Oxford Univ Press, v. 13, n. 9, p. 1193-1201, 2001.
dc.identifier.doi10.1093/intimm/13.9.1193
dc.identifier.issn0953-8178
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26613
dc.identifier.wosWOS:000171127200012
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofInternational Immunology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.subjectinflammationen
dc.subjectlymphocyteen
dc.subjectmacrophageen
dc.subjectperitoneal cellsen
dc.subjectphagocyteen
dc.subjectphagocytosisen
dc.titleMouse B-1 cell-derived mononuclear phagocyte, a novel cellular component of acute non-specific inflammatory exudateen
dc.typeinfo:eu-repo/semantics/article
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