Prebiotic and Synbiotic Modifications of Beta Oxidation and Lipogenic Gene Expression after Experimental Hypercholesterolemia in Rat Liver

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dc.citation.volume8
dc.contributor.authorAlves, Claudia Cristina [UNIFESP]
dc.contributor.authorWaitzberg, Dan Linetzky
dc.contributor.authorAndrade, Laila Santos de [UNIFESP]
dc.contributor.authorAguiar, Lais dos Santos [UNIFESP]
dc.contributor.authorReis, Milene Barcelos [UNIFESP]
dc.contributor.authorGuanabara, Camila Chaves [UNIFESP]
dc.contributor.authorAguiar, Odair [UNIFESP]
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.authorSala, Priscila
dc.coverageLausanne
dc.date.accessioned2020-08-04T13:39:49Z
dc.date.available2020-08-04T13:39:49Z
dc.date.issued2017
dc.description.abstractBackground and aims: Non-alcoholic fatty liver disease (NAFLD) is characterized by the presence of fat in hepatocytes because of decreased β-oxidation and increased lipogenesis. Prebiotics, probiotics, and synbiotic have modulatory effects on intestinal microbiota and may influence the gut-liver axis. Our aim was to evaluate the effects of prebiotic, probiotics, and synbiotic on liver histopathology and gene expression related to β-oxidation and lipogenesis after hypercholesterolemia. Methods: Wistar male adult rats (n = 40) were submitted to hypercholesterolemic conditions (HPC) (60 days). On Day 30 of HPC, rats were subdivided in 5 groups: negative control (NC): without HPC + Gv (distilled water); positive control (PC): with HPC + Gv (distilled water); prebiotic (PRE): HPC + Gv with prebiotic (Fiber FOS®); probiotic (PRO): HPC + Gv with probiotic strains Gv (Probiatop®); and synbiotic (SYN): HPC + Gv with synbiotic (Simbioflora®). All rats were sacrificed on Day 30 post-treatment. Blood was collected to verify total serum cholesterol, and liver tissue was sampled to verify histopathological changes and gene expression. Gene expression related to ß-oxidation (PPAR-α and CPT-1) and lipogenesis (SREBP-1c, FAS and ME) was evaluated in liver tissue using RT-qPCR. Results: PC had higher cholesterol levels when compared to NC. PRE and SYN rats had lower cholesterol levels than PC. PC rats showed more histopathological changes than NC rats; PRE and SYN rats showed fewer alterations than PC rats. PPAR-α was expressed at higher levels in SYN and PC rats compared with PRE and PRO rats. CPT-1 expression was similar in all groups. SREBP-1c was expressed at higher levels in PC rats compared with NC rats; levels were lower in SYN rats compared with PRO rats; levels were lower in PRE rats compared with PC and PRO rats. FAS was expressed at lower levels in PRE rats compared with SYN rats. ME expression was lower in PC rats compared with NC rats. Conclusion: Prebiotic and synbiotic supplementation improve hepatic alterations related to hypercholesterolemia. These changes appear to be mediated by altered expression of genes related to β-oxidation and lipogenesis.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Biosci, Santos, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Gastroenterol, Sch Med, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Nutr Grad, Santos, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biosci, Santos, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Nutr Grad, Santos, Brazil
dc.description.provenanceMade available in DSpace on 2020-08-04T13:39:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2017. Added 1 bitstream(s) on 2020-08-04T14:45:07Z : No. of bitstreams: 1 WOS000413105800001.pdf: 1075533 bytes, checksum: 5add3722f8ae0fc8a8e143c579171316 (MD5)en
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2011/50289-1
dc.format.extent-
dc.identifierhttps://dx.doi.org/10.3389/fmicb.2017.02010
dc.identifier.citationFrontiers In Microbiology. Lausanne, v. 8, p. -, 2017.
dc.identifier.doi10.3389/fmicb.2017.02010
dc.identifier.fileWOS000413105800001.pdf
dc.identifier.issn1664-302X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57139
dc.identifier.wosWOS:000413105800001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Microbiology
dc.rightsAcesso aberto
dc.subjectCholesterol dieten
dc.subjectSteatosisen
dc.subjectRatsen
dc.subjectPrebioticen
dc.subjectProbioticen
dc.subjectSynbioticen
dc.subjectGene expressionen
dc.titlePrebiotic and Synbiotic Modifications of Beta Oxidation and Lipogenic Gene Expression after Experimental Hypercholesterolemia in Rat Liveren
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