Can gamma-radiation modulate hemagglutinating and anticoagulant activities of PpyLL, a lectin from Phthirusa pyrifolia?

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dc.citation.volume104
dc.contributor.authorCosta, Romero M. P. B.
dc.contributor.authorCampos Albuquerque, Wendell Wagner
dc.contributor.authorSilva, Mariana C. C. [UNIFESP]
dc.contributor.authorde Paula, Raiana Apolinario
dc.contributor.authorMelo, Mychely Sheila
dc.contributor.authorOliva, Maria L. V. [UNIFESP]
dc.contributor.authorFigueiredo Porto, Ana Lucia
dc.coverageAmsterdam
dc.date.accessioned2020-09-01T13:21:29Z
dc.date.available2020-09-01T13:21:29Z
dc.date.issued2017
dc.description.abstractBlood coagulation and platelet-dependent primary homeostasis are important defense mechanisms against bleeding and novel inhibitors have been researched to obtain pharmacological and clinical applications. In this work, the PpyLL, a lectin obtained from Phthirusa pyrifolia, was characterized in terms of its molecular structure and biological functions (anticoagulant, antiplatelet agreggation and hemagglutinating activities) in presence or absence of Gamma radiation exposure. Results revealed a lectin with secondary-structure content by approximately 49% of beta-sheet, 20% of beta-turn and 31% of disordered structure. Irradiation effect demonstrated possible different sites of function by lectin on anticoagulant and hemagglutinating activities, once a decrease about 80% was observed when compared the activities under 0.5 kGy of exposition to gamma radiation. An emphatic discussion about the use of gamma radiation as a possible modulator of the lectin activity was made, and once the ionizing radiation affected differently the anticoagulation and hemagglutinating activities, we speculated that the results are determined by selective molecular damages in different binding sites. PpyLL biological activities and gamma radiation modulation could be considered for future researches in biomedical field aiming possible medical applications. (C) 2017 Elsevier B.V. All rights reserved.en
dc.description.affiliationRural Fed Univ Pernambuco UFRPE, Lab Technol Bioact Prod, Dom Manoel Medeiros St,S-N, BR-52171900 Recife, PE, Brazil
dc.description.affiliationFed Univ Pernambuco UFPE, Dept Biochem, Ctr Biol Sci, Ave Prof Moraes Rego S-N, BR-50670420 Recife, PE, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biochem, Tres Maio St,100,Vila Clementino, BR-04044020 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biochem, Tres Maio St,100,Vila Clementino, BR-04044020 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.format.extent125-136
dc.identifierhttp://dx.doi.org/10.1016/j.ijbiomac.2017.06.007
dc.identifier.citationInternational Journal Of Biological Macromolecules. Amsterdam, v. 104, p. 125-136, 2017.
dc.identifier.doi10.1016/j.ijbiomac.2017.06.007
dc.identifier.issn0141-8130
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/58295
dc.identifier.wosWOS:000412959300014
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofInternational Journal Of Biological Macromolecules
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectActivity modulateden
dc.subjectCoagulationen
dc.subjectPlatelet aggregationen
dc.subjectBinding siteen
dc.subjectGamma radiationen
dc.titleCan gamma-radiation modulate hemagglutinating and anticoagulant activities of PpyLL, a lectin from Phthirusa pyrifolia?en
dc.typeinfo:eu-repo/semantics/article
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