First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (acai) in cultured human cells

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dc.contributor.authorMarques, E. S.
dc.contributor.authorTsuboy, M. S. F.
dc.contributor.authorCarvalho, J. C. T.
dc.contributor.authorRosa, P. C. P.
dc.contributor.authorPerazzo, F. F. [UNIFESP]
dc.contributor.authorGaivao, I. O. M.
dc.contributor.authorMaistro, E. L.
dc.date.accessioned2019-08-19T11:49:42Z
dc.date.available2019-08-19T11:49:42Z
dc.date.issued2017
dc.description.abstractEuterpe oleracea Mart., popularly known as "acai", is a tropical fruit from the Amazon region where it has considerable economic importance. Acai has been used as food and for several medicinal purposes. Despite the widespread use of this fruit, there is a lack of data regarding the safety of using this fruit oil exclusively. Therefore, we evaluated the in vitro cytotoxic, genotoxic, and antigenotoxic effects of E. oleracea fruit oil (EOO) in cultured human lymphocytes (non-metabolizing cells) and HepG2 cell line (human hepatoma) (metabolizing cells) by using MTT, comet, and micronucleus assays. A wide range of EOO concentrations was tested with a preliminary MTT assay, which allowed selecting five concentrations for comet and micronucleus assays: 2.5, 10, 100, 500, and 1000 mu g/mL. The results showed that none of the EOO tested concentrations presented cytotoxic effects. The genotoxic assessment revealed an absence of significant DNA and chromosome damage in human lymphocytes and HepG2 cells but did not show chemoprotection against the DNA damage induced by methyl methanesulfonate and benzo[a] pyrene, used as DNA-damaging agents.en
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Programa Posgrad Biol Geral & Aplicada, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias & Letras, Dept Biotecnol, Assis, SP, Brazil
dc.description.affiliationUniv Fed Amapa, Colegiado Farm, Ctr Ciencias Biol & Saude, Lab Pesquisa Farmacos, Macapa, AP, Brazil
dc.description.affiliationUniv Estadual Campinas, Fac Ciencias Med, Campinas, SP, Brazil
dc.description.affiliationUniv Fed São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Exatas & Terra, Diadema, SP, Brazil
dc.description.affiliationTras Os Montes & Alto Douro Univ, CECAV, Vila Real, Portugal
dc.description.affiliationTras Os Montes & Alto Douro Univ, Dept Genet & Biotechnol, Vila Real, Portugal
dc.description.affiliationUniv Estadual Paulista, Fac Filosofia & Ciencia, Dept Fonoaudiol, Marilia, SP, Brazil
dc.description.affiliationUnifespUniv Fed São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Exatas & Terra, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP - Fundação de Amparo a Pesquisa do Estado de São Paulo
dc.description.sponsorshipCAPES
dc.description.sponsorshipIDFAPESP: 2012/17241-8
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.4238/gmr16039700
dc.identifier.citationGenetics And Molecular Research. Ribeirao Preto, v. 16, n. 3, p. -, 2017.
dc.identifier.doi10.4238/gmr16039700
dc.identifier.issn1676-5680
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/51382
dc.identifier.wosWOS:000417364500008
dc.language.isoeng
dc.publisherFunpec-Editora
dc.rightsAcesso restrito
dc.subjectAcai oilen
dc.subjectArecaceaeen
dc.subjectComet assayen
dc.subjectHepG2 cellsen
dc.subjectMicronucleus testen
dc.titleFirst cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (acai) in cultured human cellsen
dc.typeArtigo
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