Bovine papillomavirus isolation by ultracentrifugation

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dc.contributor.authorAraldi, Rodrigo Pinheiro
dc.contributor.authorSilva, Dalton Giovanni Nogueira da
dc.contributor.authorMelo, Thatiana Correa de [UNIFESP]
dc.contributor.authorDiniz, Nara
dc.contributor.authorMazzuchelli-de-Souza, Jacqueline
dc.contributor.authorSant'Ana, Thalita Araújo
dc.contributor.authorCarvalho, Rodrigo Franco de
dc.contributor.authorBeçak, Willy
dc.contributor.authorStocco, Rita de Cassia
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Fed Integracao Latino Amer
dc.date.accessioned2016-01-24T14:38:02Z
dc.date.available2016-01-24T14:38:02Z
dc.date.issued2014-11-01
dc.description.abstractThe bovine papillomavirus (BPV) is the etiological agent of bovine papillomatosis, which causes significant economic losses to livestock, characterized by the presence of papillomas that regress spontaneously or persist and progress to malignancy. Currently, there are 13 types of BPVs described in the literature as well as 32 putative new types. This study aimed to isolate viral particles of BPV from skin papillomas, using a novel viral isolation method. the virus types were previously identified with new primers designed. 77 cutaneous papilloma samples of 27 animals, Simmental breed, were surgically removed. the DNA was extracted and subjected to PCR using Delta-Epsilon and Xi primers. the bands were purified and sequenced. the sequences were analyzed using software and compared to the GenBank database, by BLAST tool. the viral typing showed a prevalence of BPV-2 in 81.81% of samples. It was also detected the presence of the putative new virus type BR/UEL2 in one sample. Virus isolation was performed by ultracentrifugation in a single density of cesium chloride. the method of virus isolation is less laborious than those previously described, allowing the isolation of complete virus particles of BPV-2. (C) 2014 Elsevier B.V. All rights reserved.en
dc.description.affiliationInst Butantan, Genet Lab, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Programa Posgrad Interunidades Biotecnol, BR-05508900 São Paulo, Brazil
dc.description.affiliationInst Butantan, Parasitol Lab, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Programa Posgrad Biol Estrutural & Func, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniv Fed Integracao Latino Amer, Dept Biol, BR-85866000 Foz Do Iguacu, PR, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Programa Posgrad Biol Estrutural & Func, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipMinisterio de Ciencia, Tecnologia e Inovacao (MCTI)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação Butantan
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDCNPq: 554816/2006-7
dc.description.sponsorshipIDCNPq: 402539/2011-7
dc.format.extent119-124
dc.identifierhttps://dx.doi.org/10.1016/j.jviromet.2014.07.029
dc.identifier.citationJournal of Virological Methods. Amsterdam: Elsevier B.V., v. 208, p. 119-124, 2014.
dc.identifier.doi10.1016/j.jviromet.2014.07.029
dc.identifier.fileWOS000343344200020.pdf
dc.identifier.issn0166-0934
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/38353
dc.identifier.wosWOS:000343344200020
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Virological Methods
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectBovine papillomavirusen
dc.subjectDiagnosisen
dc.subjectVirus isolationen
dc.subjectUltracentrifugationen
dc.titleBovine papillomavirus isolation by ultracentrifugationen
dc.typeinfo:eu-repo/semantics/article
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