Interaction between leptin and insulin signaling pathways differentially affects JAK-STAT and PI 3-kinase-mediated signaling in rat liver

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dc.contributor.authorCarvalheira, José Barreto Campello
dc.contributor.authorRibeiro, Eliane Beraldi [UNIFESP]
dc.contributor.authorFolli, Franco
dc.contributor.authorVelloso, Lício Augusto
dc.contributor.authorSaad, Mario Jose Abdalla
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:33:41Z
dc.date.available2016-01-24T12:33:41Z
dc.date.issued2003-01-01
dc.description.abstractChronic leptin treatment markedly enhances the effect of insulin on hepatic glucose production unproportionally with respect to body weight loss and increased insulin sensitivity. in the present study the crosstalk between insulin and leptin was evaluated in rat liver. Upon stimulation of JAK2 tyrosine phosphorylation, leptin induced JAK2 co-immunoprecipitation with STAT3, STAT5b, IRS-1 and IRS-2. This phenomenon parallels the leptin-induced tyrosine phosphorylation of STAT3, STAT5b, IRS-1 and IRS-2. Acutely injected insulin stimulated a mild increase in tyrosine phosphorylation of JAK2, STAT3 and STAT5b. Leptin was less effective than insulin in stimulating IRS phosphorylation and their association with PI 3-kinase. Simultaneous treatment with both hormones yielded no change in maximal phosphorylation of STAT3, IRS-1, IRS-2 and Akt, but led to a marked increase in tyrosine phosphorylation of JAK2 and STAT5b when compared with isolated administration of insulin or leptin. This indicates that there is a positive crosstalk between insulin and leptin signaling pathways at the level of JAK2 and STAT5b in rat liver.en
dc.description.affiliationUniv Estadual Campinas, FCM, Dept Clin Med, BR-13081970 Campinas, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Fisiol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Fisiol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent151-159
dc.identifierhttps://dx.doi.org/10.1515/BC.2003.016
dc.identifier.citationBiological Chemistry. Berlin: Walter de Gruyter & Co, v. 384, n. 1, p. 151-159, 2003.
dc.identifier.doi10.1515/BC.2003.016
dc.identifier.issn1431-6730
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/27112
dc.identifier.wosWOS:000180634700016
dc.language.isoeng
dc.publisherWalter de Gruyter & Co
dc.relation.ispartofBiological Chemistry
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectDNA-binding proteinsen
dc.subjectDrug effectsen
dc.subjectInsulin physiologyen
dc.subjectLeptin pharmacologyen
dc.subjectLiveren
dc.subjectMetabolismen
dc.subjectProtein-tyrosine kinaseen
dc.subjectSignal transductionen
dc.titleInteraction between leptin and insulin signaling pathways differentially affects JAK-STAT and PI 3-kinase-mediated signaling in rat liveren
dc.typeinfo:eu-repo/semantics/article
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