Antigens of Coccidioides posadasii as an Important Tool for the Immunodiagnosis of Coccidioidomycosis

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dc.contributor.authorCordeiro, Rossana de Aguiar
dc.contributor.authorNobre Patoilo, Kharla Rabelo
dc.contributor.authorPraciano, Silviane Bandeira
dc.contributor.authorAstete Medrano, Delia Jessica
dc.contributor.authorFarias Marques, Francisca Jakelyne de
dc.contributor.authorSoares Martins, Liline Maria
dc.contributor.authorEulalio, Kelsen Dantas
dc.contributor.authorDeus Filho, Antonio de
dc.contributor.authorSalmito Cavalvanti, Maria do Amparo
dc.contributor.authorBezerra Fechine, Maria Auxiliadora
dc.contributor.authorNogueira Brilhante, Raimunda Samia
dc.contributor.authorCamargo, Zoilo Pires de [UNIFESP]
dc.contributor.authorGadelha Rocha, Marcos Fabio
dc.contributor.authorCosta Sidrim, Jose Julio
dc.contributor.institutionUniv Fed Ceara
dc.contributor.institutionUniv Fed Piaui
dc.contributor.institutionInst Doencas Tropicais Natan Portella
dc.contributor.institutionUniv Integracao Int Lusofonia Afrobrasileira
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Estadual Ceara
dc.date.accessioned2016-01-24T14:31:11Z
dc.date.available2016-01-24T14:31:11Z
dc.date.issued2013-02-01
dc.description.abstractSerologic diagnosis has been presented as a safe alternative for coccidioidomycosis. However, commercial kits based on coccidioidal antibodies available in the USA are considered too expensive for laboratories outside that country. in this study, we describe the preparation of antigens for detection of human coccidioidal antibodies by the immunodiffusion test (ID) and enzyme immunoassay (EIA). Antigens were tested against serum samples from patients with coccidioidomycosis, histoplasmosis and paracoccidioidomycosis, as well as healthy individuals. the highest reactivity in the ID tests was seen in the F0-90 antigen. in the EIAs, the best results were obtained with the F60-90 antigen. None of the serum samples from healthy individuals were recognized by any of the antigen extracts tested by ID or EIA. in conclusion, the F0-90 and F60-90 antigens have the potential to be commercially employed in presumptive diagnosis of coccidioidomycosis by ID or EIA, respectively. the tests could improve serological diagnosis of coccidioidomycosis in South America.en
dc.description.affiliationUniv Fed Ceara, Postgrad Program Med Microbiol, Dept Pathol & Legal Med, Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Fed Piaui, Teresina, Piaui, Brazil
dc.description.affiliationInst Doencas Tropicais Natan Portella, Teresina, Piaui, Brazil
dc.description.affiliationUniv Integracao Int Lusofonia Afrobrasileira, Fortaleza, Ceara, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUniv Estadual Ceara, Postgrad Program Vet Sci, Fortaleza, Ceara, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipNational Council for Scientific and Technological Development (PRONEX)
dc.description.sponsorshipIDCNPq: 306637/2010-3
dc.description.sponsorshipIDNational Council for Scientific and Technological Development (PRONEX): 2155-6
dc.format.extent25-32
dc.identifierhttp://dx.doi.org/10.1007/s11046-012-9604-1
dc.identifier.citationMycopathologia. Dordrecht: Springer, v. 175, n. 1-2, p. 25-32, 2013.
dc.identifier.doi10.1007/s11046-012-9604-1
dc.identifier.issn0301-486X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35919
dc.identifier.wosWOS:000313968100003
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofMycopathologia
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectCoccidioidomycosisen
dc.subjectDiagnosisen
dc.subjectAntigenen
dc.subjectImmunodiffusionen
dc.subjectEnzyme immunoassayen
dc.titleAntigens of Coccidioides posadasii as an Important Tool for the Immunodiagnosis of Coccidioidomycosisen
dc.typeinfo:eu-repo/semantics/article
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