Paracoccidioides brasiliensis induces recruitment of alpha 3 and alpha 5 integrins into epithelial cell membrane rafts, leading to cytokine secretion

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dc.contributor.authorBarros, Bianca Carla Silva Campitelli de [UNIFESP]
dc.contributor.authorMaza, Paloma Korehisa [UNIFESP]
dc.contributor.authorAlcantara, Cristiane [UNIFESP]
dc.contributor.authorSuzuki, Erika [UNIFESP]
dc.date.accessioned2019-01-21T10:30:13Z
dc.date.available2019-01-21T10:30:13Z
dc.date.issued2016
dc.description.abstractParacoccidioides brasiliensis is one of the etiological agents of paracoccidioidomycosis, a human systemic mycosis, highly prevalent in Latin America. In the present work, we demonstrate that P. brasiliensis yeasts promote IL-6 and IL-8 secretion by the human lung epithelial cell line A549 in an integrin-dependent manner. In fact, small interfering RNA directed to alpha 3 and alpha 5 integrins decreased IL-6 and IL-8 levels in P. brasiliensis-infected A549 cell cultures. This fungus also led to an increase in the expression of alpha 3 and alpha 5 integrins in this epithelial cell line. In addition, P. brasiliensis yeasts promoted alpha 3 and alpha 5 integrins clustering into A549 cell membrane rafts. Furthermore, epithelial cell membrane raft disruption with nystatin decreased IL-6 and IL-8 levels in P. brasiliensis-A549 cell cultures. Therefore, by increasing host alpha 3 and alpha 5 integrins levels and clustering these receptors into membrane rafts, P. brasiliensis yeasts may modulate host inflammation. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.en
dc.description.affiliationDepartment of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua Botucatu, 862, 6o andar, São Paulo, SP, 04023-062, Brazil
dc.description.affiliationUnifespDepartment of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua Botucatu, 862, 6o andar, São Paulo, SP, 04023-062, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/22773-6]
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2011/22773-6
dc.format.extent68-77
dc.identifierhttps://doi.org/10.1016/j.micinf.2015.09.003
dc.identifier.citationMicrobes And Infection. Amsterdam, v. 18, n. 1, p. 68-77, 2016.
dc.identifier.doi10.1016/j.micinf.2015.09.003
dc.identifier.issn1286-4579
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/49645
dc.identifier.wosWOS:000368957800008
dc.language.isoeng
dc.publisherElsevier science bv
dc.relation.ispartofMicrobes And Infection
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMembrane Microdomainsen
dc.subjectParacoccidioidesen
dc.subjectIntegrinsen
dc.subjectInterleukin-6en
dc.subjectInterleukin-8en
dc.subjectEpithelial CellsExtracellular-Matrix Proteinsen
dc.subjectLung Adenocarcinoma Cellsen
dc.subjectSurface Proteinen
dc.subjectFibronectin Expressionen
dc.subjectMannose Receptoren
dc.subjectFungal Adhesionen
dc.subjectMapk Activationen
dc.subjectLipid Raftsen
dc.subjectIn-Vitroen
dc.subjectBindingen
dc.titleParacoccidioides brasiliensis induces recruitment of alpha 3 and alpha 5 integrins into epithelial cell membrane rafts, leading to cytokine secretionen
dc.typeinfo:eu-repo/semantics/article
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