Altered immunophenotypic features of peripheral blood platelets in myelodysplastic syndromes

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dc.contributor.authorSandes, Alex Freire [UNIFESP]
dc.contributor.authorYamamoto, Mihoko [UNIFESP]
dc.contributor.authorMatarraz, Sergio
dc.contributor.authorChauffaille, Maria de Lourdes Lopes Ferrari [UNIFESP]
dc.contributor.authorQuijano, Sandra
dc.contributor.authorLopez, Antonio
dc.contributor.authorOguro, Tsutomu
dc.contributor.authorKimura, Eliza Yuriko Sugano [UNIFESP]
dc.contributor.authorOrfao, Alberto
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Salamanca
dc.contributor.institutionHosp Serv Publ Estadual São Paulo
dc.date.accessioned2016-01-24T14:27:17Z
dc.date.available2016-01-24T14:27:17Z
dc.date.issued2012-06-01
dc.description.abstractBackgroundMultiparameter flow cytometric analysis of bone marrow and peripheral blood cells has proven to be of help in the diagnostic workup of myelodysplastic syndromes. However, the usefulness of flow cytometry for the detection of megakaryocytic and platelet dysplasia has not yet been investigated. the aim of this pilot study was to evaluate by flow cytometry the diagnostic and prognostic value of platelet dysplasia in myelodysplastic syndromes.Design and MethodsWe investigated the pattern of expression of distinct surface glycoproteins on peripheral blood platelets from a series of 44 myelodysplastic syndrome patients, 20 healthy subjects and 19 patients with platelet alterations associated to disease conditions other than myelodysplastic syndromes. Quantitative expression of CD31, CD34, CD36, CD41a, CD41b, CD42a, CD42b and CD61 glycoproteins together with the PAC-1, CD62-P, fibrinogen and CD63 platelet activation-associated markers and platelet light scatter properties were systematically evaluated.ResultsOverall, flow cytometry identified multiple immunophenotypic abnormalities on platelets of myelodysplastic syndrome patients, including altered light scatter characteristics, over-and under expression of specific platelet glycoproteins and asynchronous expression of CD34; decreased expression of CD36 (n=5), CD42a (n=1) and CD61 (n=2), together with reactivity for CD34 (n=1) were only observed among myelodysplastic syndrome cases, while other alterations were also found in other platelet disorders. Based on the overall platelet alterations detected for each patient, an immunophenotypic score was built which identified a subgroup of myelodysplastic syndrome patients with a high rate of moderate to severe alterations (score>1.5; n=16) who more frequently showed thrombocytopenia, megakaryocytic dysplasia and high-risk disease, together with a shorter overall survival.ConclusionsOur results show the presence of altered phenotypes by flow cytometry on platelets from around half of the myelodysplastic syndrome patients studied. If confirmed in larger series of patients, these findings may help refine the diagnostic and prognostic assessment of this group of disorders.en
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Disciplina Hematol & Hemoterapia, Dept Oncol Clin & Expt, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniv Salamanca, Dept Med, Serv Gen Citometria, E-37008 Salamanca, Spain
dc.description.affiliationUniv Salamanca, Canc Res Ctr IBMCC USAL CSIC, E-37008 Salamanca, Spain
dc.description.affiliationUniv Salamanca, IBSAL, E-37008 Salamanca, Spain
dc.description.affiliationHosp Serv Publ Estadual São Paulo, Serv Hematol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Disciplina Hematol & Hemoterapia, Dept Oncol Clin & Expt, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFADA (UNIFESP)
dc.description.sponsorshipInstituto de Salud Carlos III, Ministerio de Sanidad y Consumo, Madrid, Spain
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 05/57792-0
dc.description.sponsorshipIDInstituto de Salud Carlos III, Ministerio de Sanidad y Consumo, Madrid, Spain: RTICC RD06/0020/0035-FONDOS FEDER
dc.description.sponsorshipIDCNPq: 142968/2006-4
dc.description.sponsorshipIDCAPES: PDEE BEX 1025/05-8
dc.format.extent895-902
dc.identifierhttp://dx.doi.org/10.3324/haematol.2011.057158
dc.identifier.citationHaematologica-the Hematology Journal. Pavia: Ferrata Storti Foundation, v. 97, n. 6, p. 895-902, 2012.
dc.identifier.doi10.3324/haematol.2011.057158
dc.identifier.fileWOS000306066500020.pdf
dc.identifier.issn0390-6078
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34926
dc.identifier.wosWOS:000306066500020
dc.language.isoeng
dc.publisherFerrata Storti Foundation
dc.relation.ispartofHaematologica-the Hematology Journal
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectmyelodysplastic syndromesen
dc.subjectmegakaryocytic dysplasiaen
dc.subjectplateletsen
dc.subjectflow cytometryen
dc.subjectimmunophenotypingen
dc.titleAltered immunophenotypic features of peripheral blood platelets in myelodysplastic syndromesen
dc.typeinfo:eu-repo/semantics/article
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