Tooth Tissue Engineering: Optimal Dental Stem Cell Harvest Based on Tooth Development

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dc.contributor.authorDuailibi, Monica Talarico [UNIFESP]
dc.contributor.authorDuailibi, Silvio Eduardo [UNIFESP]
dc.contributor.authorDuailibi Neto, Eduardo Felippe
dc.contributor.authorNegreiros, Renata Matalon
dc.contributor.authorJorge, Waldyr Antonio
dc.contributor.authorFerreira, Lydia Masako [UNIFESP]
dc.contributor.authorVacanti, Joseph Phillip
dc.contributor.authorYelick, Pamela Crotty
dc.contributor.institutionTufts Univ
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionNatl Inst Sci & Technol
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionMassachusetts Gen Hosp
dc.contributor.institutionDept Surg
dc.contributor.institutionHarvard Univ
dc.date.accessioned2016-01-24T14:16:59Z
dc.date.available2016-01-24T14:16:59Z
dc.date.issued2011-07-01
dc.description.abstractOur long-term objective is to devise reliable methods to generate biological replacement teeth exhibiting the physical properties and functions of naturally formed human teeth. Previously, we demonstrated the successful use of tissue engineering approaches to generate small, bioengineered tooth crowns from harvested pig and rat postnatal dental stem cells (DSCs). To facilitate characterizations of human DSCs, we have developed a novel radiographic staging system to accurately correlate human third molar tooth developmental stage with anticipated harvested DSC yield. Our results demonstrated that DSC yields were higher in less developed teeth (Stages 1 and 2), and lower in more developed teeth (Stages 3, 4, and 5). the greatest cell yields and colony-forming units (CFUs) capability was obtained from Stages 1 and 2 tooth dental pulp. We conclude that radiographic developmental staging can be used to accurately assess the utility of harvested human teeth for future dental tissue engineering applications.en
dc.description.affiliationTufts Univ, Div Craniofacial & Mol Genet, Dept Oral & Maxillofacial Pathol, Boston, MA 02111 USA
dc.description.affiliationUniversidade Federal de São Paulo, Dept Plast Surg, UNIFESP CINTERGEN, Interdisciplinary Ctr Gene Therapy, São Paulo, Brazil
dc.description.affiliationNatl Inst Sci & Technol, INCT Biofabris, Biofabricat Inst, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Dent, FFO FOUSP, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Oral Maxillofacial Surg Course Dent Sch, FFO FOUSP, São Paulo, Brazil
dc.description.affiliationMassachusetts Gen Hosp, Lab Tissue Engn & Organ Fabricat, Boston, MA 02114 USA
dc.description.affiliationDept Surg, Boston, MA USA
dc.description.affiliationHarvard Univ, Sch Med, Boston, MA USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Plast Surg, UNIFESP CINTERGEN, Interdisciplinary Ctr Gene Therapy, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipNIH/NIDCR
dc.description.sponsorshipIDFAPESP: 07-58856-7
dc.description.sponsorshipIDFAPESP: 07-51227-4
dc.description.sponsorshipIDFAPESP: 07-59488-1
dc.description.sponsorshipIDNIH/NIDCR: R01DE016132
dc.description.sponsorshipIDNIH/NIDCR: R03TW007665
dc.format.extentE129-E135
dc.identifierhttp://dx.doi.org/10.1111/j.1525-1594.2010.01200.x
dc.identifier.citationArtificial Organs. Malden: Wiley-Blackwell, v. 35, n. 7, p. E129-E135, 2011.
dc.identifier.doi10.1111/j.1525-1594.2010.01200.x
dc.identifier.issn0160-564X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33878
dc.identifier.wosWOS:000292651100002
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofArtificial Organs
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectTooth developmenten
dc.subjectTooth tissue engineeringen
dc.subjectHuman dental stem cellsen
dc.subjectHuman tooth developmenten
dc.subjectTooth rapid prototypingen
dc.titleTooth Tissue Engineering: Optimal Dental Stem Cell Harvest Based on Tooth Developmenten
dc.typeinfo:eu-repo/semantics/article
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