New insights about cross-reactive epitopes of six trypanosomatid genera revealed that Crithidia and Leptomonas have antigenic similarity to L. (L.) chagasi

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dc.contributor.authorFerreira, Leandro Rodrigues
dc.contributor.authorKesper, Norival
dc.contributor.authorTeixeira, Marta M. G.
dc.contributor.authorLaurenti, Marcia Dalastra
dc.contributor.authorBarbieri, C. L. [UNIFESP]
dc.contributor.authorLindoso, Jose Angelo
dc.contributor.authorUmezawa, Eufrosina S.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInst Infectol Emilio Ribas São Paulo
dc.date.accessioned2016-01-24T14:35:22Z
dc.date.available2016-01-24T14:35:22Z
dc.date.issued2014-03-01
dc.description.abstractWe investigated whether ELISA using crude antigens from insect and plant trypanosomatids, which are non-pathogenic and easily cultivated in large scale, has the same positivity data as Leishmania (Leishmania) chagasi, the etiological agent of human visceral leishmaniasis (VL) or canine leishmaniasis (CanL), or as Trypanosoma cruzi, the etiological agent of Chagas disease (CD). the antigens from Crithidia fasciculata, Crithidia luciliae, and Leptomonas seymouri showed 100% cross-reactivity with VL and CanL samples, with no statistically titers differences from L. (L.) chagasi, however, 34% (17/50) of VL samples revealed higher titers using the insect trypanosomatids than the homologous antigen. On the other hand, antigens from Strigomonas culicis, Angomonas deanei, and Phytomonas serpens showed low cross-reactivity with VL and CanL samples. the sera from patients with American tegumentary leishmaniasis showed low levels of cross-reactivity with all trypanosomatids investigated, even with L. (L) chagasi, without titers dissimilarity among them. These parasites were also worthless as antigen source for detection of CD cases, which required homologous antigens to reach 100% positivity. This study showed, by ELISA, that crude extract of Crithidia and Leptomonas have epitopes similar to L. (L.) chagasi, which supports the idea of using them as antigens source for the serodiagnosis of visceral leishmaniasis. Published by Elsevier B.V.en
dc.description.affiliationUniv São Paulo, Inst Med Trop Salo Paulo, BR-09500900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Dept Prevent Med, BR-09500900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-09500900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Dept Patol, BR-09500900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, São Paulo, Brazil
dc.description.affiliationInst Infectol Emilio Ribas São Paulo, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipLIM49-FMUSP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent41-46
dc.identifierhttp://dx.doi.org/10.1016/j.actatropica.2013.11.010
dc.identifier.citationActa Tropica. Amsterdam: Elsevier B.V., v. 131, p. 41-46, 2014.
dc.identifier.doi10.1016/j.actatropica.2013.11.010
dc.identifier.issn0001-706X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37483
dc.identifier.wosWOS:000332905500007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofActa Tropica
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectL. (L.) chagasien
dc.subjectTrypanosoma cruzien
dc.subjectCrithidiaen
dc.subjectLeptomonasen
dc.subjectTrypanosomatidsen
dc.subjectCross-reactivityen
dc.titleNew insights about cross-reactive epitopes of six trypanosomatid genera revealed that Crithidia and Leptomonas have antigenic similarity to L. (L.) chagasien
dc.typeinfo:eu-repo/semantics/article
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