Antifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazil

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dc.contributor.authorFigueiredo, Thais Pandini
dc.contributor.authorde Lucas, Rosymar Coutinho
dc.contributor.authorCazzaniga, Rodrigo Anselmo
dc.contributor.authorFranca, Carolina Nunes [UNIFESP]
dc.contributor.authorSegato, Fernando
dc.contributor.authorTaglialegna, Rafael
dc.contributor.authorMaffei, Cláudia Maria Leite
dc.date.accessioned2019-01-21T10:29:31Z
dc.date.available2019-01-21T10:29:31Z
dc.date.issued2016
dc.description.abstractCryptococcosis is a leading invasive fungal infection in immunocompromised patients. Considering the high prevalence and severity of these infections in immunocompromised patients attended at HC-FMRP-USP, the present research aimed to characterize the clinical isolates of Cryptococcus strains by biochemical and molecular methods and evaluate antifungal susceptibility of clinical isolates. Fifty isolates from 32 HIV-positive patients were obtained at HC-FMRP-USP. Most of the isolates (78.1%) were identified as C. neoformans, and 100% of C. neoformans and C. gattii strains were susceptible to amphotericin B, ketoconazole and fluconazole. All isolates were classified as serotype A (grubbii variety) by PCR and most of them were characterized in mating type MATa. PCR analysis of specific M13 microsatellite sequence revealed that VNI type was predominant among C. neoformans, while VGII was predominant among C. gattii. The strains did not show a significant resistance to the antifungals tested, and Canavanine-Glycine-Bromthymol Blue Agar (CGB) proved to be a reliable test presenting a good correlation with the molecular characterization. C. neoformans isolated from disseminated infections in the same patient showed molecular identity when different anatomical sites were compareden
dc.description.abstractbesides, the studied strains did not present a significant increase in resistance to antifungal agents. In addition, the homogeneity of the molecular types and detection of the mating types suggested a low possibility of crossing among the strains.en
dc.description.affiliationUniversidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Clínica Médica. 14049-900 Ribeirão Preto, SP, Brazil
dc.description.affiliationUniversidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Bioquímica. 14049-900 Ribeirão Preto, SP, Brazil
dc.description.affiliationUniversidade de São Paulo, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Departamento de Biologia. 14040-901 Ribeirão Preto, SP, Brazil
dc.description.affiliationUniversidade Federal de Sergipe, Departamento de Morfologia. 49100-000, SE, Brazil
dc.description.affiliationUniversidade de Santo Amaro, UNISA, Pós Graduação em Ciências da Saúde, São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Departamento de Medicina, São Paulo, SP, Brazil
dc.description.affiliationEscola de Engenharia de Lorena, Departamento de Biotecnologia. 12602-810, Lorena, SP,Universidade de São Paulo Brazil
dc.description.affiliationUniversidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Biologia Celular, Molecular e Bioagentes Patogênicos. 14049-900 Ribeirão Preto, SP, Brazil
dc.description.affiliationUniversidade Federal de Ouro Preto, Núcleo de Inovação Tecnológica, 35400-000 Ouro Preto, MG, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Departamento de Medicina, São Paulo, SP, Brazil.
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordination for the Improvement of Higher Education Personnel, Brazil - (CAPES - Institutional Quota)
dc.format.extentUNSP 69
dc.identifierhttp://dx.doi.org/10.1590/S1678-9946201658069
dc.identifier.citationRevista Do Instituto De Medicina Tropical De Sao Paulo. Sao paulo, v. 58, p. UNSP 69, 2016.
dc.identifier.doi10.1590/S1678-9946201658069
dc.identifier.fileS0036-46652016005000253.pdf
dc.identifier.issn0036-4665
dc.identifier.scieloS0036-46652016005000253
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/49254
dc.identifier.wosWOS:000396241000020
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofRevista Do Instituto De Medicina Tropical De Sao Paulo
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCryptococcus Neoformansen
dc.subjectSerotypingen
dc.subjectGenotypingen
dc.subjectFingerprintingen
dc.subjectAntifungal Agentsen
dc.subjectMating Typeen
dc.subjectHiv-PatientsPolymerase-Chain-Reactionen
dc.subjectSpecies Complexen
dc.subjectMating-Typesen
dc.subjectSerotype-Aen
dc.subjectMolecular Characterizationen
dc.subjectVar. Gattiien
dc.subjectStrainsen
dc.subjectVirulenceen
dc.subjectDnaen
dc.subjectPathogenesisen
dc.titleAntifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazilen
dc.typeinfo:eu-repo/semantics/article
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