Co-administration of alpha-GalCer analog and TLR4 agonist induces robust CD8(+) T-cell responses to PyCS protein and WT-1 antigen and activates memory-like effector NKT cells

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dc.citation.volume168
dc.contributor.authorCoelho-dos-Reis, Jordana G.
dc.contributor.authorHuang, Jing
dc.contributor.authorTsao, Tiffany
dc.contributor.authorPereira, Felipe V. [UNIFESP]
dc.contributor.authorFunakoshi, Ryota
dc.contributor.authorNakajima, Hiroko
dc.contributor.authorSugiyama, Haruo
dc.contributor.authorTsuji, Moriya
dc.coverageSan Diego
dc.date.accessioned2020-08-14T13:44:25Z
dc.date.available2020-08-14T13:44:25Z
dc.date.issued2016
dc.description.abstractIn the present study, the combined adjuvant effect of 7DW8-5, a potent alpha-GalCer-analog, and monophosphoryl lipid A (MPLA), a TLR4 agonist, on the induction of vaccine-induced CD8(+) T-cell responses and protective immunity was evaluated. Mice were immunized with peptides corresponding to the CD8+ T-cell epitopes of a malaria antigen, a circumsporozoite protein of Plasmodium yoelff, and a tumor antigen, a Wilms Tumor antigen-1 (WT-1), together with 7DW8-5 and MPLA, as an adjuvant. These immunization regimens were able to induce higher levels of CD8+ T-cell responses and, ultimately, enhanced levels of protection against malaria and tumor challenges compared to the levels induced by immunization with peptides mixed with 7DW8-5 or MPLA alone. Co-administration of 7DW8-5 and MPLA induces activation of memory-like effector natural killer T (NKT) cells, i.e. CD44(+) CD62L(-) NKT cells. Our study indicates that 7DW8-5 greatly enhances important synergistic pathways associated to memory immune responses when co-administered with MPLA, thus rendering this combination of adjuvants a novel vaccine adjuvant formulation. (C) 2016 Elsevier Inc. All rights reserved.en
dc.description.affiliationRockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10016 USA
dc.description.affiliationFundacao Oswaldo Cruz FIOCRUZ, Ctr Pesquisas Rene Rachou, BR-30192 Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Sao Paulo, BR-04021 Sao Paulo, Brazil
dc.description.affiliationOsaka Univ, Grad Sch Med, Dept Canc Immunol, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan
dc.description.affiliationOsaka Univ, Grad Sch Med, Dept Funct Diagnost Sci, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan
dc.description.affiliationUnifespUniv Fed Sao Paulo, BR-04021 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipNIH
dc.description.sponsorshipIDNIH: AI070258
dc.format.extent6-15
dc.identifierhttp://dx.doi.org/10.1016/j.clim.2016.04.014
dc.identifier.citationClinical Immunology. San Diego, v. 168, p. 6-15, 2016.
dc.identifier.doi10.1016/j.clim.2016.04.014
dc.identifier.issn1521-6616
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57659
dc.identifier.wosWOS:000380297600002
dc.language.isoeng
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.ispartofClinical Immunology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAdjuvanten
dc.subjectGlycolipiden
dc.subjectNKT cellsen
dc.subjectCD1den
dc.subjectTLR4en
dc.subjectMalaria vaccineen
dc.subjectCancer vaccineen
dc.subjectWT-1en
dc.subjectCircumsporozoite proteinen
dc.subjectMemory-like effector NKT cellsen
dc.titleCo-administration of alpha-GalCer analog and TLR4 agonist induces robust CD8(+) T-cell responses to PyCS protein and WT-1 antigen and activates memory-like effector NKT cellsen
dc.typeinfo:eu-repo/semantics/article
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