Vascular Therapy for Radiation Cystitis

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dc.contributor.authorSoler, Roberto [UNIFESP]
dc.contributor.authorVianello, Alberto
dc.contributor.authorFuellhase, Claudius
dc.contributor.authorWang, Zhan
dc.contributor.authorAtala, Anthony
dc.contributor.authorSoker, Shay
dc.contributor.authorYoo, James J.
dc.contributor.authorWilliams, James Koudy
dc.contributor.institutionWake Forest Univ Hlth Sci
dc.contributor.institutionLudwig Maximilians Univ Munchen
dc.contributor.institutionUniv Perugia
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:05:56Z
dc.date.available2016-01-24T14:05:56Z
dc.date.issued2011-01-01
dc.description.abstractPurpose: the underlying pathology of radiation cystitis is cellular and vascular damage followed by increased fibrosis and inflammation. This study was to determine if neovascular- promoting therapy could reduce the pathological changes in the bladder wall associated with pelvic irradiation. Methods: Adult female Lewis inbred rats were irradiated with a single dose of 20 Gy directed at their bladder. Four weeks later, 30 rats were divided equally into one of three treatment groups for bladder wall injection of: (1) PBS (Control); (2) PBS containing 50 ng vascular endothelial growth factor (VEGF165); or (3) PBS containing 1 x 10(6) rat endothelial cells (EC). Age- matched non- irradiated rats (n 10) served as untreated controls. At either 1.5 or 3 months following radiation, bladders were analyzed for collagen deposition using Masson's Trichrome staining of collagen and muscle and vascularization using Von Willebrand factor staining of ECs. Quantitative- PCR was used to examine markers of angiogenesis, hypoxia, and fibrosis. Results: the collagen/ muscle ratio was doubled in the control group 3months post- irradiation (P < 0.05 vs. non- irradiated bladders). Both ECs and VEGF inhibited increases in collagen content (P < 0.05 vs. control). Similarly, irradiation reduced bladder wall vessel counts compared to non- irradiated controls (P < 0.05) and both ECs and VEGF maintained vessel counts similar to that of non- irradiated controls (P < 0.05). PCR analysis showed a higher expression of neovascular markers (CD31, KDR) in the EC and VEGF groups compared to non- irradiated controls (P < 0.05). Conclusions: Angiogenesis therapy may be useful in the prevention and/or treatment of the underlying pathology of radiation cystitis. Neurourol. Urodynam. 30: 428- 434, 2011. (C) 2010 Wiley-Liss, Inc.en
dc.description.affiliationWake Forest Univ Hlth Sci, Inst Regenerat Med, Winston Salem, NC USA
dc.description.affiliationWake Forest Univ Hlth Sci, Dept Urol, Winston Salem, NC USA
dc.description.affiliationLudwig Maximilians Univ Munchen, Univ Hosp Grosshadern, Dept Urol, Munich, Germany
dc.description.affiliationUniv Perugia, Dept Med Surg Specialties & Publ Hlth, Urol Sect, I-06100 Perugia, Italy
dc.description.affiliationUniversidade Federal de São Paulo, Div Urol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Urol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipRadiation Cystitis Association
dc.format.extent428-434
dc.identifierhttp://dx.doi.org/10.1002/nau.21002
dc.identifier.citationNeurourology and Urodynamics. Malden: Wiley-Blackwell, v. 30, n. 3, p. 428-434, 2011.
dc.identifier.doi10.1002/nau.21002
dc.identifier.issn0733-2467
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33255
dc.identifier.wosWOS:000288398400031
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofNeurourology and Urodynamics
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectcollagenen
dc.subjectendothelial cellsen
dc.subjectneovascularizationen
dc.subjectradiationen
dc.subjectradiation cystitisen
dc.subjectratsen
dc.subjectVEGFen
dc.titleVascular Therapy for Radiation Cystitisen
dc.typeinfo:eu-repo/semantics/article
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