Melatonin modifies basal and stimulated insulin secretion via NADPH oxidase

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dc.citation.issue3
dc.citation.volume231
dc.contributor.authorSimoes, Daniel
dc.contributor.authorRiva, Patricia
dc.contributor.authorPeliciari-Garcia, Rodrigo Antonio [UNIFESP]
dc.contributor.authorCruzat, Vinicius Fernandes
dc.contributor.authorGraciano, Maria Fernanda
dc.contributor.authorMunhoz, Ana Claudia
dc.contributor.authorTaneda, Marco
dc.contributor.authorCipolla-Neto, Jose
dc.contributor.authorCarpinelli, Angelo Rafael
dc.coverageBristol
dc.date.accessioned2020-07-31T12:47:14Z
dc.date.available2020-07-31T12:47:14Z
dc.date.issued2016
dc.description.abstractMelatonin is a hormone synthesized in the pineal gland, which modulates several functions within the organism, including the synchronization of glucose metabolism and glucose-stimulated insulin secretion (GSIS). Melatonin can mediate different signaling pathways in pancreatic islets through two membrane receptors and via antioxidant or pro-oxidant enzymes modulation. NADPH oxidase (NOX) is a pro-oxidant enzyme responsible for the production of the reactive oxygen specie (ROS) superoxide, generated from molecular oxygen. In pancreatic islets, NOX-derived ROS can modulate glucose metabolism and regulate insulin secretion. Considering the roles of both melatonin and NOX in islets, the aim of this study was to evaluate the association of NOX and ROS production on glucose metabolism, basal and GSIS in pinealectomized rats (PINX) and in melatonin-treated isolated pancreatic islets. Our results showed that ROS content derived from NOX activity was increased in PINX at baseline (2.8 mM glucose), which was followed by a reduction in glucose metabolism and basal insulin secretion in this group. Under 16.7 mM glucose, an increase in both glucose metabolism and GSIS was observed in PINX islets, without changes in ROS content. In isolated pancreatic islets from control animals incubated with 2.8 mM glucose, melatonin treatment reduced ROS content, whereas in 16.7 mM glucose, melatonin reduced ROS and GSIS. In conclusion, our results demonstrate that both basal and stimulated insulin secretion can be regulated by melatonin through the maintenance of ROS homeostasis in pancreatic islets.en
dc.description.affiliationUniv Sao Paulo, Inst Biomed Sci 1, Dept Physiol & Biophys, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biol Sci, Lab Biosyst, Sao Paulo, Brazil
dc.description.affiliationUnifespDepartment of Biological Sciences, Laboratory of Biosystems, Universidade Federal de São Paulo (UNIFESP), Diadema, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2013/08769-1
dc.format.extent235-244
dc.identifierhttp://dx.doi.org/10.1530/JOE-16-0259
dc.identifier.citationJournal Of Endocrinology. Bristol, v. 231, n. 3, p. 235-244, 2016.
dc.identifier.doi10.1530/JOE-16-0259
dc.identifier.issn0022-0795
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56681
dc.identifier.wosWOS:000387982200007
dc.language.isoeng
dc.publisherBioscientifica Ltd
dc.relation.ispartofJournal Of Endocrinology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectmelatoninen
dc.subjectNADPH oxidaseen
dc.subjectsuperoxideen
dc.subjectglucose metabolismen
dc.subjectROSen
dc.titleMelatonin modifies basal and stimulated insulin secretion via NADPH oxidaseen
dc.typeinfo:eu-repo/semantics/article
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