Parylene scaffold for cartilage lesion

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dc.citation.issue2
dc.citation.volume19
dc.contributor.authorda Silveira Franciozi, Carlos Eduardo [UNIFESP]
dc.contributor.authorVangsness, Carleton Thomas, Jr.
dc.contributor.authorMartinez, Juan Carlos
dc.contributor.authorRodger, Damien
dc.contributor.authorChou, Tzu-Chieh
dc.contributor.authorTai, Yu-Chong
dc.contributor.authorBrant, Rodrigo [UNIFESP]
dc.contributor.authorWu, Ling
dc.contributor.authorAbdalla, Rene Jorge [UNIFESP]
dc.contributor.authorHan, Bo
dc.contributor.authorEvseenko, Denis
dc.contributor.authorHumayun, Mark
dc.coverageDordrecht
dc.date.accessioned2020-07-13T11:53:11Z
dc.date.available2020-07-13T11:53:11Z
dc.date.issued2017
dc.description.abstractEvaluate parylene scaffold feasibility in cartilage lesion treatment, introducing a novel paradigm combining a reparative and superficial reconstructive procedure. Fifteen rabbits were used. All animals had both knees operated and the same osteochondral lesion model was created bilaterally. The parylene scaffold was implanted in the right knee, and the left knee of the same animal was used as control. The animals were euthanized at different time points after surgery: four animals at three weeks, three animals at six weeks, four animals at nine weeks, and four animals at 12 weeks. Specimens were analyzed by International Cartilage Repair Society (ICRS) macroscopic evaluation, modified Pineda histologic evaluation of cartilage repair, and collagen II immunostaining. Parylene knees were compared to its matched contra-lateral control knees of the same animal using the Wilcoxon matched-pairs signed rank. ICRS mean +/- SD values for parylene versus control, three, six, nine and twelve weeks, respectively: 7.83 +/- 1.85 versus 4.42 +/- 1.08, p = 0.0005en
dc.description.abstract10.17 +/- 1.17 versus 6.83 +/- 1.17, p = 0.03en
dc.description.abstract10.89 +/- 0.60 versus 7.33 +/- 2.18, p = 0.007en
dc.description.abstract10.67 +/- 0.78 versus 7.83 +/- 3.40, p = 0.03. Modified Pineda mean +/- SD values for parylene versus control, six, nine and twelve weeks, respectively: 3.37 +/- 0.87 versus 6.94 +/- 1.7, p < 0.0001en
dc.description.abstract5.73 +/- 2.05 versus 6.41 +/- 1.7, p = 0.007en
dc.description.abstract3.06 +/- 1.61 versus 6.52 +/- 1.51, p < 0.0001. No inflammation was seen. Parylene implanted knees demonstrated higher collagen II expression via immunostaining in comparison to the control knees. Parylene scaffolds are a feasible option for cartilage lesion treatment and the combination of a reparative to a superficial reconstructive procedure using parylene scaffolds led to better results than the reparative procedure alone.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Orthopaed & Traumatol, Rua Borges Lagoa 783,5th Floor, BR-04038032 Sao Paulo, Brazil
dc.description.affiliationUniv Southern Calif, Dept Orthopaed Surg, 1520 San Pablo St,Suite 2000, Los Angeles, CA 90033 USA
dc.description.affiliationUniv Southern Calif, Dept Ophthalmol, USC Roski Eye Inst, Los Angeles, CA 90033 USA
dc.description.affiliationUniv Southern Calif, USC Inst Biomed Therapeut, 1450 San Pablo St,Room 6534, Los Angeles, CA 90033 USA
dc.description.affiliationCALTECH, Dept Elect Engn, 1200 E Calif Blvd,Mail Code 136-93, Pasadena, CA 91125 USA
dc.description.affiliationUniv Fed Sao Paulo, Dept Ophthalmol, Rua Botucatu 821, BR-04023062 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Southern Calif, Dept Orthopaed Surg, 2011 Zonal Ave,HMR 712, Los Angeles, CA 90033 USA
dc.description.affiliationInVitro Cell Res LLC, Englewood, NJ 07631 USA
dc.description.affiliationUniv Southern Calif, Dept Surg, 1450 San Pablo St,Healthcare Consultat Ctr 4, Los Angeles, CA 90089 USA
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Orthopaed & Traumatol, Rua Borges Lagoa 783,5th Floor, BR-04038032 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Ophthalmol, Rua Botucatu 821, BR-04023062 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipVangsness Research Fund from Keck School of Medicine - University of Southern California
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP
dc.description.sponsorshipIDFAPESP: 2015/08952-6
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1007/s10544-017-0170-7
dc.identifier.citationBiomedical Microdevices. Dordrecht, v. 19, n. 2, p. -, 2017.
dc.identifier.doi10.1007/s10544-017-0170-7
dc.identifier.issn1387-2176
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54440
dc.identifier.wosWOS:000400547000008
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofBiomedical Microdevices
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectBiomaterialen
dc.subjectTissue engineeringen
dc.subjectScaffolden
dc.subjectParyleneen
dc.subjectCartilageen
dc.subjectCartilage treatmenten
dc.titleParylene scaffold for cartilage lesionen
dc.typeinfo:eu-repo/semantics/article
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