Protein expression of VEGF, IGF-1 and FGF in retroocular connective tissues and clinical correlation in Graves' ophthalmopathy

Página do item simplificado
dc.contributor.authorMatos, Kimble [UNIFESP]
dc.contributor.authorManso, Paulo Gois [UNIFESP]
dc.contributor.authorMarback, Eduardo [UNIFESP]
dc.contributor.authorFurlanetto, Reinaldo [UNIFESP]
dc.contributor.authorAlberti, Gustave Nosé
dc.contributor.authorNosé, Vania [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal da Bahia
dc.contributor.institutionTufts University Medical Center
dc.contributor.institutionBrigham and Women's Hospital Department of Pathology
dc.date.accessioned2015-06-14T13:38:40Z
dc.date.available2015-06-14T13:38:40Z
dc.date.issued2008-08-01
dc.description.abstractPURPOSE: To investigate the immunohistochemical expression (IGF-1, EGFr, EGF, c-erbB-2/HER-2/neu, PDGF-A, PDGF-B, FGF and VEGF) in patients with Graves' ophthalmopathy. METHODS: Twenty-four samples (Graves' ophthalmopathy patients) underwent lateral rectus muscle and surrounding fibrous and adipose tissue biopsy. The control group was obtained by strabismus surgery. Correlation between clinical- ophthalmologic, endocrinological, ultrasonographic findings, and immunohistochemical expression was performed. RESULTS: IGF-1: There were 7 positive cases (29.2%). There was a direct relation with higher CAS (clinical activity score) in all of them and if only CAS equal or higher than 5 was considered, this was 54.5%. FGF: There was expression in 5 cases (20.8%) with a direct relation in all those with higher CAS (>5) (45.4%). VEGF: There were two positive cases (8.3%) for VEGF in endothelial cells, in these cases the patients also presented CAS higher than 5. There was no expressions of all growth factors in the control group. CONCLUSIONS: All patients, except one, with positive expression of FGF, IGF-1 and VEGF showed CAS greater than 5, suggesting in this way an important role of these growth factors in the pathogenesis and severity of Graves' ophthalmopathy. However, statistical analysis revealed only significant association between IGF-1 and male sex (P=0.034). Low ultrasound reflectivity and endocrine status may not correlate directly with disease activity or with immunoexpression of growth factors and c-erbB-2/HER-2/neu.en
dc.description.abstractOBJETIVO: Investigar a expressão imuno-histoquímica de IGF-1, EGFr, EGF, c-erbB-2/HER-2/neu, PDGF-A, PDGF-B, FGF e VEGF na oftalmopatia de Graves. MÉTODOS: Vinte e dois pacientes (oftalmopatia de Graves) foram submetidos à biópsia do músculo reto lateral e tecido fibroso e adiposo adjacente. O grupo controle foi de pacientes de cirurgia de estrabismo. Foi feita correlação entre achados clínico-oftalmológicos, endocrinológicos, ultra-sonográficos e da expressão imuno-histoquímica dos fatores de crescimento. RESULTADOS: IGF-1: Houve 7 casos positivos (29,2%). Houve correlação direta com o CAS (clinical activity score) elevado em todos os casos e em que consideramos CAS apenas acima de 5, em 54,5%. FGF: Houve expressão em 5 casos (20,8%) com relação direta com CAS elevado em todos os casos e em que consideramos CAS maior que 5 (45,4%). VEGF: Houve dois casos positivos (8,3%) para VEGF nas células endoteliais e estes casos também apresentavam CAS maior que 5. A imunorreatividade foi negativa em todo grupo controle. CONCLUSÃO: Todos os pacientes, com exceção de um, com expressão positiva para FGF, IGF-1 e VEGF mostraram CAS maior que 5, sugerindo importante papel destes fatores de crescimento na patogênese e gravidade da oftalmopatia de Graves. Entretanto, a análise estatística demonstrou associação significativa entre IGF-1 e o sexo masculino (P=0,034). Baixa refletividade ao ultra-som e condição endócrina não estiveram correlacionadas.pt
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Department of Ophtalmology
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Department of Ophtalmology
dc.description.affiliationUniversidade Federal da Bahia
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Department of Endocrinology
dc.description.affiliationTufts University Medical Center
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Department of Pathology
dc.description.affiliationBrigham and Women's Hospital Department of Pathology
dc.description.affiliationUnifespUNIFESP, Department of Ophtalmology
dc.description.affiliationUnifespUNIFESP, Department of Ophtalmology
dc.description.affiliationUnifespUNIFESP, Department of Endocrinology
dc.description.affiliationUnifespUNIFESP, Department of Pathology
dc.description.sourceSciELO
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent486-492
dc.identifierhttp://dx.doi.org/10.1590/S0004-27492008000400004
dc.identifier.citationArquivos Brasileiros de Oftalmologia. Conselho Brasileiro de Oftalmologia, v. 71, n. 4, p. 486-492, 2008.
dc.identifier.doi10.1590/S0004-27492008000400004
dc.identifier.fileS0004-27492008000400004.pdf
dc.identifier.issn0004-2749
dc.identifier.scieloS0004-27492008000400004
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/4497
dc.language.isoeng
dc.publisherConselho Brasileiro de Oftalmologia
dc.relation.ispartofArquivos Brasileiros de Oftalmologia
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectImunohistochemstryen
dc.subjectReceptor, erbB-2en
dc.subjectInsulin-like growth factor Ien
dc.subjectReceptor, epidermal growth factoren
dc.subjectEpidermal growth factoren
dc.subjectPlatelet-derived growth factor alphaen
dc.subjectProto-oncogene proteins c-sisen
dc.subjectFibroblast growth factorsen
dc.subjectVascular endothelial growth factor Aen
dc.subjectGraves' ophthalmopathyen
dc.subjectImuno-histoquímicapt
dc.subjectReceptor erbB-2pt
dc.subjectFator de crescimento insulin-like Ipt
dc.subjectReceptor do fator de crescimento epidérmicopt
dc.subjectFator de crescimento epidérmicopt
dc.subjectReceptor tipo alfa para fator de crescimento derivado de plaquetapt
dc.subjectProteínas proto-oncogênicas c-sispt
dc.subjectFatores de crescimento de fibroblastospt
dc.subjectFator A de crescimento do endotélio vascularpt
dc.subjectOftalmopatia de Gravespt
dc.titleProtein expression of VEGF, IGF-1 and FGF in retroocular connective tissues and clinical correlation in Graves' ophthalmopathyen
dc.title.alternativeExpressão protéica de VEGF, IGF-1 e FGF no tecido conjuntivo retro-ocular e correlação clínica na oftalmopatia de Gravespt
dc.typeinfo:eu-repo/semantics/article
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
S0004-27492008000400004.pdf
Tamanho:
492.05 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
EPM - Artigos