Conjugated polymers nanostructured as smart interfaces for controlling the catalytic properties of enzymes

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dc.contributor.authorBarbosa, Camila Gouveia [UNIFESP]
dc.contributor.authorCaseli, Luciano [UNIFESP]
dc.contributor.authorPeres, Laura Oliveira [UNIFESP]
dc.date.accessioned2019-07-22T15:46:54Z
dc.date.available2019-07-22T15:46:54Z
dc.date.issued2016
dc.description.abstractThe search for new molecular architectures to improve the efficiency of enzymes entrapped in ultrathin films is useful to enhance the effectiveness of biosensors. In this present work, conjugated polymers, based on thiophene and fluorine, were investigated to verify their suitability as matrices for the immobilization of urease. The copolymer poly[(9,9-dioctylfluorene)-co-thiophene], PDOF-co-Th was spread on the air-water interface forming stable Langmuir monolayers as determined by surface pressure-area isotherms, polarization-modulation reflection-absorption infrared spectroscopy (PM-IRRAS), and Brewster angle microscopy (BAM). Urease was incorporated in the floating monolayers being further transferred to solid supports as mixed Langmuir-Blodgett (LB) films. These films were then characterized with transfer ratio, fluorescence spectroscopy, PM-IRRAS and atomic force microscopy, confirming the co-transfer of the enzyme as well as its structuring in beta-sheets. The catalytic activity was detected for urease, with a lower reaction rate than that encountered for the homogeneous environment. This was attributed to conformational constraints imposed to the biomacromolecule entrapped in the polymeric matrix. (C) 2016 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniv Fed Sao Paulo, Lab Hybrid Mat, Diadema, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Lab Hybrid Mat, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP [2015/23446-0]
dc.description.sponsorshipCNPq [401109/2014-3]
dc.description.sponsorshipCAPES
dc.description.sponsorshipIDFAPESP:2015/23446-0
dc.description.sponsorshipIDCNPq:401109/2014-3
dc.format.extent206-213
dc.identifierhttp://dx.doi.org/10.1016/j.jcis.2016.05.033
dc.identifier.citationJournal Of Colloid And Interface Science. San Diego, v. 476, p. 206-213, 2016.
dc.identifier.doi10.1016/j.jcis.2016.05.033
dc.identifier.issn0021-9797
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/51168
dc.identifier.wosWOS:000378370100023
dc.language.isoeng
dc.publisherAcademic Press Inc Elsevier Science
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectConjugated polymeren
dc.subjectUreaseen
dc.subjectLangmuir-Blodgetten
dc.subjectEnzyme activityen
dc.subjectBiosensoren
dc.titleConjugated polymers nanostructured as smart interfaces for controlling the catalytic properties of enzymesen
dc.typeinfo:eu-repo/semantics/article
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