Medial Ganglionic Eminence Cells Freshly Obtained or Expanded as Neurospheres Show Distinct Cellular and Molecular Properties in Reducing Epileptic Seizures

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dc.citation.issue2
dc.citation.volume23
dc.contributor.authorRomariz, Simone A. A. [UNIFESP]
dc.contributor.authorPaiva, Daisylea S. [UNIFESP]
dc.contributor.authorGalindo, Layla T. [UNIFESP]
dc.contributor.authorBarnabe, Gabriela F.
dc.contributor.authorGuedes, Vivian A.
dc.contributor.authorBorlongan, Cesario V.
dc.contributor.authorLongo, Beatriz M. [UNIFESP]
dc.coverageHoboken
dc.date.accessioned2020-07-17T14:03:08Z
dc.date.available2020-07-17T14:03:08Z
dc.date.issued2017
dc.description.abstractAimsMedial ganglionic eminence (MGE) progenitors give rise to inhibitory interneurons and may serve as an alternative cell source for large-scale cell transplantation for epilepsy after in vitro expansion. We investigated whether modifications in the culture medium of MGE neurospheres affect neuronal differentiation and expression of MGE-specific genes. In vivo, we compared anticonvulsant effects and cell differentiation pattern among neurospheres grown in different culture media and compared them with freshly harvested MGE cells. MethodsWe used four variations of cell culture: standard, containing growth factors (EGF/FGF-2) (GF)en
dc.description.abstractaddition of retinoic acid (GF-RA)en
dc.description.abstractwithdrawal of EGF/FGF-2 (WD)en
dc.description.abstractand addition of retinoic acid and withdrawal of EGF/FGF-2 (WD-RA). Based on in vitro results neurosphere-grown (WD-RA or GF conditions) or fresh MGE cells were transplanted into the hippocampus. ResultsIn vitro WD-RA showed increased neuronal population and higher expression of Dlx1, Nkx2.1, and Lhx6 genes in comparison with GF culture condition. After transplantation, fresh MGE cells and neurospheres (GF) showed anticonvulsant effects. However, fresh MGE cells differentiated preferentially into inhibitory neurons, while GF gave rise to glial cells. ConclusionWe conclude that freshly isolated and neurosphere-grown MGE cells reduced seizures by different mechanisms (inhibitory interneurons vs. astrocytes). Fresh MGE cells appear more appropriate for cell therapies targeting inhibitory interneurons for conferring anticonvulsant outcomes.en
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Dept Fisiol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Dept Bioquim, Sao Paulo, SP, Brazil
dc.description.affiliationLudwig Inst Canc Res, Inst Sirio Libanes Ensino & Pesquisa, Sao Paulo, SP, Brazil
dc.description.affiliationUniv S Florida, Dept Neurosurg & Brain Repair, Tampa, FL USA
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Dept Fisiol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Dept Bioquim, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP
dc.description.sponsorshipCNPq
dc.format.extent127-134
dc.identifierhttp://dx.doi.org/10.1111/cns.12650
dc.identifier.citationCns Neuroscience & Therapeutics. Hoboken, v. 23, n. 2, p. 127-134, 2017.
dc.identifier.doi10.1111/cns.12650
dc.identifier.issn1755-5930
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55186
dc.identifier.wosWOS:000394157900003
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofCns Neuroscience & Therapeutics
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectEpilepsyen
dc.subjectInterneuronal progenitorsen
dc.subjectMedial ganglionic eminenceen
dc.subjectNeurospheresen
dc.subjectTransplantationen
dc.titleMedial Ganglionic Eminence Cells Freshly Obtained or Expanded as Neurospheres Show Distinct Cellular and Molecular Properties in Reducing Epileptic Seizuresen
dc.typeinfo:eu-repo/semantics/article
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