2,3-Di-O-sulfo glucuronic acid: An unmodified and unusual residue in a highly sulfated chondroitin sulfate from Litopenaeus vannamei

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dc.citation.volume183
dc.contributor.authorCavalcante, Romulo S.
dc.contributor.authorBrito, Adriana S.
dc.contributor.authorPalhares, Lais C. G. F.
dc.contributor.authorLima, Marcelo A. [UNIFESP]
dc.contributor.authorCavalheiro, Renan P. [UNIFESP]
dc.contributor.authorNader, Helena B. [UNIFESP]
dc.contributor.authorSassaki, Guilherme L.
dc.contributor.authorChavante, Suely F.
dc.coverageOxford
dc.date.accessioned2020-07-08T13:09:36Z
dc.date.available2020-07-08T13:09:36Z
dc.date.issued2018
dc.description.abstractThe occurrence of a natural and unmodified highly sulfated chondroitin sulfate from Litopenaeus vannamei heads (sCS) is herein reported. Its partial digestion by Chondroitinases AC and ABC together with its electrophoretic migration profile revealed it as a highly sulfated chondroitin sulfate despite its average molecular weight being similar to CSA. Using orthogonal 1D/2D NMR experiments, the anomeric signals (delta 4.62/106.0) corresponding to unusual 2,3-di-O-Sulfo-GlcA (similar to 36%), U3(3S) (delta 4.42/84.1, similar to 63%) and U2(2S) (4.12/80.1, similar to 50%) substitutions were confirmed. In addition, non-sulfated GlcA (delta 4.5/106.3) linked to 4-O-(A1(4S), 36%) or 6-O-Sulfo (A1(6S), 28%) GalNAc (delta 4.64/103.5) was observed. Although the biological role of sCS in shrimp is unknown, its influence on hemostasis was also demonstrated. The sCS identification brings to light new questions about the hierarchical model of GAGs biosynthesis and contributes to the better understanding of the subtle relationship between GAGs structure and function.en
dc.description.affiliationUniv Fed Rio Grande do Norte, Dept Bioquim, Programa Posgrad Bioquim & Biol Mol, Natal, RN, Brazil
dc.description.affiliationUniv Fed Rio Grande do Norte, Dept Morfol, Programa Posgrad Ciencias Saude, Natal, RN, Brazil
dc.description.affiliationUniv Fed Rio Grande do Norte, Fac Ciencias Saude Trairi, Santa Cruz, RN, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Bioquim, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Liverpool, Dept Biochem, Liverpool L69 7ZB, Merseyside, England
dc.description.affiliationUniv Fed Parana, Dept Bioquim & Biol Mol, Curitiba, PR, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Bioquim, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCNPq (Conselho Nacional de Desenvolvimento e Tecnologico) [456160/2014-0]
dc.description.sponsorshipCAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
dc.description.sponsorshipFAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo), Brazil
dc.description.sponsorshipUFPR NMR Center
dc.description.sponsorshipCAPES
dc.format.extent192-200
dc.identifierhttp://dx.doi.org/10.1016/j.carbpol.2017.12.018
dc.identifier.citationCarbohydrate Polymers. Oxford, v. 183, p. 192-200, 2018.
dc.identifier.doi10.1016/j.carbpol.2017.12.018
dc.identifier.issn0144-8617
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54096
dc.identifier.wosWOS:000423715000020
dc.language.isoeng
dc.publisherElsevier Sci Ltd
dc.relation.ispartofCarbohydrate Polymers
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChondroitin sulfateen
dc.subjectNMR spectroscopyen
dc.subjectStructural caracterizationen
dc.subjectLitopenaeus vannameien
dc.title2,3-Di-O-sulfo glucuronic acid: An unmodified and unusual residue in a highly sulfated chondroitin sulfate from Litopenaeus vannameien
dc.typeinfo:eu-repo/semantics/article
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