Expression and Distribution of Kinin B-2 Receptor in Medulla and Spinal Cord Tissues of Rats Treated with Capsaicin

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dc.citation.issue4
dc.citation.volume34
dc.contributor.authorFranca, Camilla Estevao de [UNIFESP]
dc.contributor.authorGuardabassi, Carla [UNIFESP]
dc.contributor.authorSmith, Ricardo Luiz [UNIFESP]
dc.coverageTemuco
dc.date.accessioned2020-07-31T12:47:05Z
dc.date.available2020-07-31T12:47:05Z
dc.date.issued2016
dc.description.abstractKinins are vasoactive peptides that promote pain and inflammation, yet centrally believed to participate to cardiovascular defensive reflexes produced by noxious stimuli. These peptides signal through the activation of two transmembrane Gprotein-coupled receptors named B-1 and B-2 receptors (B1R and B2R). The B2R is constitutive in healthy tissues and animals. The aim of the study was to measure the gene and protein expression of B2R kinin receptors in central and peripheral tissues isolated from control rats and rats were pre-treated with capsaicin on the second day of life (50 mg/kg, s.c.) or two weeks prior to sacrifice (125 mg/kg over three days, s.c.). The same treatment with saline was made in control animals. Levels of mRNA for B2R were measured by quantitative RT-PCR and Qualitative while receptor binding sites were measured on tissue sections with the radioligands 125I-HPP-Hoe 140 (B2R). B2R was expressed in all studied tissues (hypothalamus, paratrigeminal nucleus, nucleus of solitary tract, spinal cord, aorta and liver) and treatment capsaicin neonates when compared to controls, did not affect its level of expression. Capsaicin had no significant effect on the expression of B2R in some tissues on binding sites. The synthesis of B2R kinin receptor is not associated with sensory C-fibre and tissues showed no significant difference indicating that B2R was regulated by distinct mechanisms.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Morphol & Genet, Sao Paulo, Brazil
dc.description.affiliationCanadian Inst Hlth Res, CNPq, Ottawa, ON, Canada
dc.description.affiliationUnifespDepartment of Morphology and Genetics, Universidade Federal de São Paulo, Brasi
dc.description.sourceWeb of Science
dc.format.extent1465-1471
dc.identifierhttp://dx.doi.org/10.4067/S0717-95022016000400047
dc.identifier.citationInternational Journal Of Morphology. Temuco, v. 34, n. 4, p. 1465-1471, 2016.
dc.identifier.doi10.4067/S0717-95022016000400047
dc.identifier.fileS0717-95022016000400047.pdf
dc.identifier.issn0717-9502
dc.identifier.scieloS0717-95022016000400047
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56570
dc.identifier.wosWOS:000395669600047
dc.language.isoeng
dc.publisherSoc Chilena Anatomia
dc.relation.ispartofInternational Journal Of Morphology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMedullaen
dc.subjectSpinal Corden
dc.subjectRatsen
dc.subjectKinin receptor B-2en
dc.subjectCapsaicinen
dc.titleExpression and Distribution of Kinin B-2 Receptor in Medulla and Spinal Cord Tissues of Rats Treated with Capsaicinen
dc.typeinfo:eu-repo/semantics/article
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