Inflammasome gene profile is modulated in septic patients, with a greater magnitude in non-survivors

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dc.contributor.authorEsquerdo, K. F. [UNIFESP]
dc.contributor.authorSharma, N. K. [UNIFESP]
dc.contributor.authorBrunialti, M. K. C. [UNIFESP]
dc.contributor.authorBaggio-Zappia, G. L. [UNIFESP]
dc.contributor.authorAssuncao, M.
dc.contributor.authorAzevedo, L. C. P.
dc.contributor.authorBafi, A. T. [UNIFESP]
dc.contributor.authorSalomao, R. [UNIFESP]
dc.date.accessioned2019-08-19T11:50:14Z
dc.date.available2019-08-19T11:50:14Z
dc.date.issued2017
dc.description.abstractInflammasome signalling induces the processing and secretion of interleukin (IL)-1 and IL-18 which, coupled with pyroptosis, activate further the inflammatory response. In the present study we evaluated the expression of genes involved in inflammasome signalling pathways in septic patients, their interaction networks and the predicted functions modulated in survivors and non-survivors. Twenty-seven patients with sepsis secondary to community-acquired pneumonia admitted to intensive care units from three general hospitals in SAo Paulo were included into the study. We performed a polymerase chain reaction (PCR) array encompassing 35 genes related to the nucleotide-binding oligomerization domain and leucine-rich repeat-containing (NLR)-inflammasome in peripheral blood mononuclear cells obtained at admission and after 7 days of follow-up. Eleven healthy volunteers were used as the reference group. Increased NLRC4 and NLRP3 and decreased nucleotide-binding oligomerization domain (NOD1), and NLRP1 expression was observed in septic patients compared to healthy individualsen
dc.description.abstractthe IL-1 and IL-18 expression levels were also high in the patients. The gene expression changes followed the same patterns in surviving and non-surviving patients, with higher magnitudes observed in non-survivors. Functional analyses revealed, however, that activation and inhibition intensity for representing functions were different in survivors and non-survivors, as for production of reactive oxygen species, synthesis of nitric oxide and for the control of bacterial infections. Our results showed that the genes involved in the activation of the NLR-inflammasome cascades were altered substantially in septic patients, with a higher number of altered genes and a higher intensity in the disturbance of gene expression found among patients dying of sepsis.en
dc.description.affiliationUniv Fed São Paulo, Hosp São Paulo, Escola Paulista Med, São Paulo, SP, Brazil
dc.description.affiliationHosp Israelita Albert Einstein, São Paulo, Brazil
dc.description.affiliationHosp Sirio Libanes, São Paulo, Brazil
dc.description.affiliationUnifespUniv Fed São Paulo, Hosp São Paulo, Escola Paulista Med, São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
dc.description.sponsorshipIDFAPESP: 2011/20401-4
dc.description.sponsorshipIDCNPq: 305685/2011-2
dc.format.extent232-240
dc.identifierhttp://dx.doi.org/10.1111/cei.12971
dc.identifier.citationClinical And Experimental Immunology. Hoboken, v. 189, n. 2, p. 232-240, 2017.
dc.identifier.doi10.1111/cei.12971
dc.identifier.issn0009-9104
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/51495
dc.identifier.wosWOS:000405634300011
dc.language.isoeng
dc.publisherWiley
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectinterleukin-18en
dc.subjectinterleukin-1betaen
dc.subjectNLRP3en
dc.subjectpyroptosisen
dc.subjectToll-like receptoren
dc.titleInflammasome gene profile is modulated in septic patients, with a greater magnitude in non-survivorsen
dc.typeinfo:eu-repo/semantics/article
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