Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique

Página do item simplificado
dc.contributor.authorSilva, Reinaldo Correia [UNIFESP]
dc.contributor.authorFaiçal, Samir [UNIFESP]
dc.contributor.authorLaureti, Stefano
dc.contributor.authorFalorni, Alberto
dc.contributor.authorDib, Sergio Atala [UNIFESP]
dc.contributor.authorKater, Claudio Elias [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversity of Perugia
dc.date.accessioned2015-06-14T13:24:46Z
dc.date.available2015-06-14T13:24:46Z
dc.date.issued1998-09-01
dc.description.abstractAdrenocortical autoantibodies (ACA), present in 60-80% of patients with idiopathic Addison's disease, are conventionally detected by indirect immunofluorescence (IIF) on frozen sections of adrenal glands. The large-scale use of IIF is limited in part by the need for a fluorescence microscope and the fact that histological sections cannot be stored for long periods of time. To circumvent these restrictions we developed a novel peroxidase-labelled protein A (PLPA) technique for the detection of ACA in patients with Addison's disease and compared the results with those obtained with the classical IIF assay. We studied serum samples from 90 healthy control subjects and 22 patients with Addison's disease, who had been clinically classified into two groups: idiopathic (N = 13) and granulomatous (N = 9). ACA-PLPA were detected in 10/22 (45%) patients: 9/13 (69%) with the idiopathic form and 1/9 (11%) with the granulomatous form, whereas ACA-IIF were detected in 11/22 patients (50%): 10/13 (77%) with the idiopathic form and 1/9 (11%) with the granulomatous form. Twelve of the 13 idiopathic addisonians (92%) were positive for either ACA-PLPA or ACA-IIF, but only 7 were positive by both methods. In contrast, none of 90 healthy subjects was found to be positive for ACA. Thus, our study shows that the PLPA-based technique is useful, has technical advantages over the IIF method (by not requiring the use of a fluorescence microscope and by permitting section storage for long periods of time). However, since it is only 60% concordant with the ACA-IIF method, it should be considered complementary instead of an alternative method to IIF for the detection of ACA in human sera.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniversity of Perugia
dc.description.affiliationUnifespUNIFESP
dc.description.sourceSciELO
dc.format.extent1141-1148
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X1998000900007
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 9, p. 1141-1148, 1998.
dc.identifier.doi10.1590/S0100-879X1998000900007
dc.identifier.fileS0100-879X1998000900007.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X1998000900007
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/674
dc.identifier.wosWOS:000076297100007
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsAcesso aberto
dc.subjectautoantibodies detectionen
dc.subjectAddison's diseaseen
dc.subjectperoxidase-labelled protein Aen
dc.subjectindirect immunofluorescenceen
dc.subjectadrenal glanden
dc.titleDetection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A techniqueen
dc.typeArtigo
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
S0100-879X1998000900007.pdf
Tamanho:
56.71 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
Em verificação - Geral