A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a(1), MSA-2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-gamma producing cells

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dc.citation.volume9
dc.contributor.authorGimenez, Alba Marina [UNIFESP]
dc.contributor.authorFrancoso, Katia S.
dc.contributor.authorErsching, Jonatan [UNIFESP]
dc.contributor.authorIcimoto, Marcelo Yudi [UNIFESP]
dc.contributor.authorOliveira, Vitor [UNIFESP]
dc.contributor.authorRodriguez, Anabel E.
dc.contributor.authorSchnittger, Leonhard
dc.contributor.authorFlorin-Christensen, Monica
dc.contributor.authorRodrigues, Mauricio Martins [UNIFESP]
dc.contributor.authorSoares, Irene S.
dc.coverageLondon
dc.date.accessioned2020-07-31T12:47:17Z
dc.date.available2020-07-31T12:47:17Z
dc.date.issued2016
dc.description.abstractBackground: Babesia bovis is a tick transmitted protozoan hemoparasite and the causative agent of bovine babesiosis, a potential risk to more than 500 million cattle worldwide. The vaccines currently available are based on attenuated parasites, which are difficult to produce, and are only recommended for use in bovines under one year of age. When used in older animals, these vaccines may cause life-threatening clinical symptoms and eventually death. The development of a multi-subunit recombinant vaccine against B. bovis would be attractive from an economic standpoint and, most importantly, could be recommended for animals of any age. In the present study, recombinant ectodomains of MSA-2a(1), MSA-2b and MSA-2c antigens were expressed in Pichia pastoris yeast as secreted soluble peptides. Results: The antigens were purified to homogeneity, and biochemically and immunologically characterized. A vaccine formulation was obtained by emulsifying a mixture of the three peptides with the adjuvant Montanide ISA 720, which elicited high IgG antibody titers against each of the above antigens. IgG antibodies generated against each MSA-antigen recognized merozoites and significantly inhibited the invasion of bovine erythrocytes. Cellular immune responses were also detected, which were characterized by splenic and lymph node CD4(+) T cells producing IFN-gamma and TNF-alpha upon stimulation with the antigens MSA-2a(1) or MSA-2c. Conclusions: These data strongly suggest the high protective potential of the presented formulation, and we propose that it could be tested in vaccination trials of bovines challenged with B. bovis.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, CTCMOL, Escola Paulista Med, Rua Mirassol 207, BR-04044010 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, BR-05508900 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biofis, BR-04023062 Sao Paulo, Brazil
dc.description.affiliationINTA Castelar, Inst Patobiol, CICVyA, RA-1686 Hurlingham, Argentina
dc.description.affiliationConsejo Nacl Invest Cient & Tecn, C1033AAJ Ciudad Autonoma Buenos Aires, RA-1033 Buenos Aires, DF, Argentina
dc.description.affiliationUnifespCTCMOL, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo-Escola Paulista de Medicina, Rua Mirassol, 207, São Paulo, 04044-010, SP, Brazil
dc.description.affiliationUnifespDepartamento de Biofísica, Universidade Federal de São Paulo, CEP 04023-062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)/Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.sponsorshipInstituto Nacional de Ciencia e Tecnologia de Vacinas (INCTV-CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipAgencia Nacional de Promocion Cientifica y Tecnologica, Argentina
dc.description.sponsorshipInstituto Nacional de Tecnologia Agropecuaria, INTA, Argentina
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDCNPq/CONICET: 490395/2011-2
dc.description.sponsorshipIDAgencia Nacional de Promocion Cientifica y Tecnologica, Argentina: PICT 2010-0438
dc.description.sponsorshipIDInstituto Nacional de Tecnologia Agropecuaria, INTA, Argentina: PNBIO 1131034
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1186/s13071-016-1862-1
dc.identifier.citationParasites & Vectors. London, v. 9, p. -, 2016.
dc.identifier.doi10.1186/s13071-016-1862-1
dc.identifier.fileWOS000388145600001.pdf
dc.identifier.issn1756-3305
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56717
dc.identifier.wosWOS:000388145600001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofParasites & Vectors
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBabesia bovisen
dc.subjectMerozoitesen
dc.subjectRecombinant vaccineen
dc.titleA recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a(1), MSA-2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-gamma producing cellsen
dc.typeinfo:eu-repo/semantics/article
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