Analysis of the functional aspects and seminal plasma proteomic profile of sperm from smokers

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dc.citation.issue5
dc.citation.volume118
dc.contributor.authorAntoniassi, Mariana Pereira [UNIFESP]
dc.contributor.authorIntasqui, Paula [UNIFESP]
dc.contributor.authorCamargo, Mariana [UNIFESP]
dc.contributor.authorZylbersztejn, Daniel Suslik [UNIFESP]
dc.contributor.authorCarvalho, Valdemir Melechco
dc.contributor.authorCardozo, Karina H. M.
dc.contributor.authorBertolla, Ricardo Pimenta [UNIFESP]
dc.coverageHoboken
dc.date.accessioned2020-07-31T12:47:29Z
dc.date.available2020-07-31T12:47:29Z
dc.date.issued2016
dc.description.abstractObjective To evaluate the effect of smoking on sperm functional quality and seminal plasma proteomic profile. Patients and Methods Sperm functional tests were performed in 20 non-smoking men with normal semen quality, according to the World Health Organization (2010) and in 20 smoking patients. These included: evaluation of DNA fragmentation by alkaline Comet assayen
dc.description.abstractanalysis of mitochondrial activity using DAB stainingen
dc.description.abstractand acrosomal integrity evaluation by PNA binding. The remaining semen was centrifuged and seminal plasma was used for proteomic analysis (liquid chromatographytandem mass spectrometry). The quantified proteins were used for Venn diagram construction in Cytoscape 3.2.1 software, using the PINA4MS plug-in. Then, differentially expressed proteins were used for functional enrichment analysis of Gene Ontology categories, Kyoto Encyclopedia of Genes and Genomes and Reactome, using Cytoscape software and the ClueGO 2.2.0 plug-in. Results Smokers had a higher percentage of sperm DNA damage (Comet classes III and IVen
dc.description.abstractP < 0.01), partially and fully inactive mitochondria (DAB classes III and IVen
dc.description.abstractP = 0.001 and P = 0.006, respectively) and non-intact acrosomes (P < 0.01) when compared with the control group. With respect to proteomic analysis, 422 proteins were identified and quantified, of which one protein was absent, 27 proteins were under-represented and six proteins were over-represented in smokers. Functional enrichment analysis showed the enrichment of antigen processing and presentation, positive regulation of prostaglandin secretion involved in immune response, protein kinase A signalling and arachidonic acid secretion, complement activation, regulation of the cytokinemediated signalling pathway and regulation of acute inflammatory response in the study group (smokers). Conclusion In conclusion, cigarette smoking was associated with an inflammatory state in the accessory glands and in the testis, as shown by enriched proteomic pathways. This state causes an alteration in sperm functional quality, which is characterized by decreased acrosome integrity and mitochondrial activity, as well as by increased nuclear DNA fragmentation.en
dc.description.affiliationUniv Fed Sao Paulo, Div Urol, Human Reprod Sect, Dept Surg, Sao Paulo, Brazil
dc.description.affiliationFleury Grp, Sao Paulo, Brazil
dc.description.affiliationHosp Sao Paulo, Sao Paulo, Brazil
dc.description.affiliationUnifespDepartment of Surgery, Division of Urology, Human Reproduction Section, Universidade Federal de São Paulo (UNIFESP), Sao Paulo, Brazil
dc.description.affiliationUnifespHospital Sao Paulo, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFleury Projects Funding (Fleury Group)
dc.description.sponsorshipIDCNPq: 160857/2012-0
dc.description.sponsorshipIDCNPq: 306616/2013-0
dc.format.extent814-822
dc.identifierhttp://dx.doi.org/10.1111/bju.13539
dc.identifier.citationBju International. Hoboken, v. 118, n. 5, p. 814-822, 2016.
dc.identifier.doi10.1111/bju.13539
dc.identifier.issn1464-4096
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56856
dc.identifier.wosWOS:000386468600025
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofBju International
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectacrosomeen
dc.subjectDNA damageen
dc.subjectmitochondriaen
dc.subjectproteomicsen
dc.subjectseminal plasmaen
dc.titleAnalysis of the functional aspects and seminal plasma proteomic profile of sperm from smokersen
dc.typeinfo:eu-repo/semantics/article
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