Study of acute chagasic mice under immunosuppressive therapy by cyclosporin A: modulation and confocal analysis of inflammatory reaction

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dc.contributor.authorCalabrese, K. S.
dc.contributor.authorParadela, ASRC
dc.contributor.authorValle, T. Z. do
dc.contributor.authorTedesco, R. C.
dc.contributor.authorSilva, S.
dc.contributor.authorMortara, R. A.
dc.contributor.authorCosta, SCG da
dc.contributor.institutionIst Oswaldo Cruz
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:31:03Z
dc.date.available2016-01-24T12:31:03Z
dc.date.issued2000-04-01
dc.description.abstractThe in vivo effects of cyclosporin A (CsA) on Trypanosoma cruzi infection were examined using different schedules of the drug in mice infected with the Y strain. Parasitaemia at day 8 after infection among CsA-treated animals was usually higher than control infected non-treated mice. On the other hand, mortality analysis showed that animals CsA-treated either with 200 mg/kg 2 days before infection or with therapeutic doses (10 mg/kg every other day) showed almost the same mean time of death (35.8 and 38.2 days, respectively). in these groups mice died 50% less than control infected non-treated ones. the mean time of death in the animals treated with 200 mg/kg 5 days after infection and in infected non-treated control mice were respectively 29.0 and 22.6 days. the kinetics analysis of the leukocyte population of animals treated with a single dose of 200 mg/kg of CsA before or after infection did not show the alternate pattern of leukopenia/leukocytosis observed in control groups of infected mice but differential cell counts indicated a modulatory action upon circulating leukocytes of therapeutic doses of CsA. the animals treated with any of the CsA schedules showed a moderate to intense diffuse inflammatory reaction exhibiting mainly mononuclear cells in the heart. Immunofluorescence analysis by confocal microscopy revealed that macrophages are a major component of the inflammatory infiltrate in all groups of CsA-treated mice and also in the control group. (C) 2000 Elsevier Science B.V. All rights reserved.en
dc.description.affiliationIst Oswaldo Cruz, Dept Protozool, Lab Imunomodulacao, BR-21045900 Rio de Janeiro, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1-11
dc.identifierhttp://dx.doi.org/10.1016/S0162-3109(99)00179-4
dc.identifier.citationImmunopharmacology. Amsterdam: Elsevier B.V., v. 47, n. 1, p. 1-11, 2000.
dc.identifier.doi10.1016/S0162-3109(99)00179-4
dc.identifier.issn0162-3109
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26280
dc.identifier.wosWOS:000086010200001
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofImmunopharmacology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectimmunosuppressionen
dc.subjectcyclosporin Aen
dc.subjectacute Chagas' diseaseen
dc.subjectTrypanosoma cruzien
dc.subjectinflammatory reactionen
dc.subjectmurine modelen
dc.subjectconfocal microscopyen
dc.titleStudy of acute chagasic mice under immunosuppressive therapy by cyclosporin A: modulation and confocal analysis of inflammatory reactionen
dc.typeinfo:eu-repo/semantics/article
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