CdSe magic-sized quantum dots incorporated in biomembrane models at the air-water interface composed of components of tumorigenic and non-tumorigenic cells

Página do item simplificado
dc.citation.issue7
dc.citation.volume1858
dc.contributor.authorGoto, Thiago E. [UNIFESP]
dc.contributor.authorLopes, Carla C. [UNIFESP]
dc.contributor.authorNader, Helena B. [UNIFESP]
dc.contributor.authorSilva, Anielle C. A.
dc.contributor.authorDantas, Noeli O.
dc.contributor.authorSiqueira, Jose R., Jr.
dc.contributor.authorCaseli, Luciano [UNIFESP]
dc.coverageAmsterdam
dc.date.accessioned2020-08-14T13:44:32Z
dc.date.available2020-08-14T13:44:32Z
dc.date.issued2016
dc.description.abstractCadmium selenide (CdSe) magic-sized quantum dots (MSQDs) are semiconductor nanocrystals with stable luminescence that are feasible for biomedical applications, especially for in vivo and in vitro imaging of tumor cells. In this work, we investigated the specific interaction of CdSe MSQDs with tumorigenic and non-tumorigenic cells using Langmuir monolayers and Langmuir-Blodgett (LB) films of lipids as membrane models for diagnosis of cancerous cells. Surface pressure-area isotherms and polarization modulation reflection-absorption spectroscopy (PM-IRRAS) showed an intrinsic interaction between the quantum dots, inserted in the aqueous subphase, and Langmuir monolayers constituted either of selected lipids or of tumorigenic and non-tumorigenic cell extracts. The films were transferred to solid supports to obtain microscopic images, providing information on their morphology. Similarity between films with different compositions representing cell membranes, with or without the quantum dots, was evaluated by atomic force microscopy (AFM) and confocal microscopy. This study demonstrates that the affinity of quantum dots for models representing cancer cells permits the use of these systems as devices for cancer diagnosis. (C) 2016 Elsevier B.V. All rights reserved.en
dc.description.affiliationFed Univ Silo Paulo UNIFESP, Inst Environm Chem & Pharmaceut Sci ICAQF, BR-09913030 Diadema, SP, Brazil
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Dept Biochem, BR-04039032 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Uberlandia, Inst Phys, Lab New Insulator & Semicond Mat LNMIS, BR-38400902 Uberlandia, MG, Brazil
dc.description.affiliationFed Univ TrianguloMineiro UFIM, Inst Exact Sci Nat & Educ, BR-38064200 Uberaba, MG, Brazil
dc.description.affiliationUnifespFed Univ Silo Paulo UNIFESP, Inst Environm Chem & Pharmaceut Sci ICAQF, BR-09913030 Diadema, SP, Brazil
dc.description.affiliationUnifespFed Univ Sao Paulo UNIFESP, Dept Biochem, BR-04039032 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCNPq
dc.description.sponsorshipFAPEMIG
dc.description.sponsorshipFAPESP
dc.description.sponsorshipIDCNPq: 477668/2013-5
dc.description.sponsorshipIDCNPq: 470890/2012-6
dc.description.sponsorshipIDFAPEMIG: APQ-01763-13
dc.description.sponsorshipIDFAPESP: 2015/23446-0
dc.format.extent1533-1540
dc.identifierhttp://dx.doi.org/10.1016/j.bbamem.2016.04.009
dc.identifier.citationBiochimica Et Biophysica Acta-Biomembranes. Amsterdam, v. 1858, n. 7, p. 1533-1540, 2016.
dc.identifier.doi10.1016/j.bbamem.2016.04.009
dc.identifier.issn0005-2736
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57719
dc.identifier.wosWOS:000377233000013
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofBiochimica Et Biophysica Acta-Biomembranes
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCdSe magic-sized quantum dotsen
dc.subjectTumorigenic and non-tumorigenic cellsen
dc.subjectLangmuir-Blodgett ultrathin filmsen
dc.subjectCancer diagnosisen
dc.titleCdSe magic-sized quantum dots incorporated in biomembrane models at the air-water interface composed of components of tumorigenic and non-tumorigenic cellsen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
ICAQF - Artigos
EPM - Artigos