Characterization of Enterococcus species isolated from marine recreational waters by MALDI-TOF MS and Rapid ID API (R) 20 Strep system

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dc.citation.issue1/fev
dc.citation.volume118
dc.contributor.authorGuarnieri Christ, Ana Paula
dc.contributor.authorRamos, Solange Rodrigues
dc.contributor.authorCayo, Rodrigo [UNIFESP]
dc.contributor.authorGales, Ana Cristina [UNIFESP]
dc.contributor.authorHachich, Elayse Maria
dc.contributor.authorZanoli Sato, Maria Ines
dc.coverageOxford
dc.date.accessioned2020-07-13T11:53:13Z
dc.date.available2020-07-13T11:53:13Z
dc.date.issued2017
dc.description.abstractMALDI-TOF Mass Spectrometry Biotyping has proven to be a reliable method for identifying bacteria at the species level based on the analysis of the ribosomal proteins mass fingerprint. We evaluate the usefulness of this method to identify Enterococcus species isolated from marine recreational water at Brazilian beaches. A total of 127 Enterococcus spp. isolates were identified to species level by bioMerieux's API (R) 20 Strep and MALDI-TOF systems. The biochemical test identified 117/127 isolates (92%), whereas MALDI identified 100% of the isolates, with an agreement of 63% between the methods. The 165 rRNA gene sequencing of isolates with discrepant results showed that MALDI-TOF and API (R) correctly identified 74% and 11% of these isolates, respectively. This discrepancy probably relies on the bias of the API (R) has to identify clinical isolates. MALDI-TOF proved to be a feasible approach for identifying Enterococcus from environmental matrices increasing the rapidness and accuracy of results. (C) 2017 Elsevier Ltd. All rights reserved.en
dc.description.affiliationCETESB Companhia Ambiental Estado Sao Paulo, Dept Environm Anal, Sao Paulo, SP, Brazil
dc.description.affiliationUNIFESP Univ Fed Sao Paulo, Dept Med, ALERTA Lab, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUNIFESP Univ Fed Sao Paulo, Dept Med, ALERTA Lab, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCETESB - Environmental Company of sao Paulo State
dc.description.sponsorshipCNPq - National Council for Science and Technological Development
dc.description.sponsorshipIDCNPq: 305535/2014-5
dc.format.extent376-381
dc.identifierhttp://dx.doi.org/10.1016/j.marpolbul.2017.03.025
dc.identifier.citationMarine Pollution Bulletin. Oxford, v. 118, n. 43497, p. 376-381, 2017.
dc.identifier.doi10.1016/j.marpolbul.2017.03.025
dc.identifier.issn0025-326X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54458
dc.identifier.wosWOS:000402217300056
dc.language.isoeng
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofMarine Pollution Bulletin
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectEnterococcien
dc.subjectSpecies identificationen
dc.subjectGram-positiveen
dc.subjectBeachesen
dc.subject16S rRNA gene sequencingen
dc.subjectMALDI-TOF MSen
dc.titleCharacterization of Enterococcus species isolated from marine recreational waters by MALDI-TOF MS and Rapid ID API (R) 20 Strep systemen
dc.typeinfo:eu-repo/semantics/article
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