Molecular and mycotoxigenic biodiversity of Aspergillus flavus isolated from Brazil nuts

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dc.citation.volume89
dc.contributor.authorBaquiao, Arianne Costa
dc.contributor.authorLopes, Evandro Luiz [UNIFESP]
dc.contributor.authorCorrea, Benedito
dc.coverageAmsterdam
dc.date.accessioned2020-07-31T12:47:25Z
dc.date.available2020-07-31T12:47:25Z
dc.date.issued2016
dc.description.abstractThe objective of this study was to carry out a transcription analysis of eight genes belonging to the aflatoxin (AF) and cyclopiazonic acid (CPA) biosynthesis pathway, and to detect aflatoxin B-1 (AFB(1)) and CPA production in Aspergillus flavus strains isolated from Brazil nuts. Additionally, these genes were correlated with the different mycotoxigenic profiles of the same strains. Four previously identified A. flavus strains (ICB-01, ICB-151, ICB-161, and ICB-165) were grown on Brazil nut agar at 25 degrees C for 10 days. Mycotoxins were separated by high-performance liquid chromatography. Transcriptional analysis was performed by real-time RT-PCR using specific primers designed based on the conserved regions of two regulatory genes (aflR and aflS), three structural genes of the AFB(1) biosynthesis pathway (aflH, aflJ and aflP), and three structural genes of the CPA biosynthesis pathway (maoA, dmaT and pks-nrps). The expression of most genes in the A. flavus isolates varied according to the mycotoxin profile of each strain. The most expressed genes in the aflatoxigenic strain ICB-151 were aflJ (77.11%) and aflH (32.75%), while the CPA-producing strain ICB-161 mainly expressed dmaT (100%), maoA (63.72%), aflS (43.52%), and aflR (42.63%). The ICB-01 isolate was a producer of AFB(1) and CPA and the most expressed genes were aflS (47.79%), dmaT (42.77%), aflP (39.5%), and aflR (38.02%). ICB-198 did not produce any mycotoxin and exhibited lower expression of almost all genes analyzed. Furthermore, the ratio of aflS/aflR expression was correlated with the biosynthesis of AF and CPA in A. flavus strains producing exclusively AF or CPA or producing both AF and CPA. The ratio of aflS/aflR expression therefore seems to be related to the production of mycotoxins in Brazil nuts. Our results provide important data for the development of innovative and more cost-effective strategies to reduce and prevent AFB and CPA contamination in Brazil nuts. (C) 2016 Published by Elsevier Ltd.en
dc.description.affiliationUniv Sao Paulo, Inst Ciencias Biomed 2, Dept Microbiol, Av Prof Lineu Prestes 1374, BR-05508000 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Polit Econ & Negocios, Sao Paulo, Brazil
dc.description.affiliationUnifespEscola Paulista de Política, Economia e Negócios, Universidade Federal de São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent266-271
dc.identifierhttp://dx.doi.org/10.1016/j.foodres.2016.08.005
dc.identifier.citationFood Research International. Amsterdam, v. 89, p. 266-271, 2016.
dc.identifier.doi10.1016/j.foodres.2016.08.005
dc.identifier.issn0963-9969
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56821
dc.identifier.wosWOS:000388775500024
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofFood Research International
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAflatoxinen
dc.subjectAspergillus flavusen
dc.subjectaflRen
dc.subjectCyclopiazonic aciden
dc.subjectdmaTen
dc.subjectGene expressionen
dc.titleMolecular and mycotoxigenic biodiversity of Aspergillus flavus isolated from Brazil nutsen
dc.typeinfo:eu-repo/semantics/article
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