Growth and microcystin production of a Brazilian Microcystis aeruginosa strain (LTPNA 02) under different nutrient conditions

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dc.contributor.authorBortoli, Stella
dc.contributor.authorOliveira-Silva, Diogo [UNIFESP]
dc.contributor.authorKrüger, Thomas
dc.contributor.authorDörr, Felipe A.
dc.contributor.authorColepicolo, Pio
dc.contributor.authorVolmer, Dietrich A.
dc.contributor.authorPinto, Ernani
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionSaarland University Institute of Bioanalytical Chemistry
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFriedrich-Schiller University of Jena Institute of Nutrition
dc.contributor.institutionHans Knöll Institute Leibniz Institute for Natural Product Research and Infection Biology
dc.date.accessioned2015-06-14T13:47:13Z
dc.date.available2015-06-14T13:47:13Z
dc.date.issued2014-08-01
dc.description.abstractCyanobacteria are prokaryotic and photosynthetic organisms, which can produce a wide range of bioactive compounds with different properties; including a variety of toxic compounds, also known as cyanotoxins. In this work, we describe the isolation of seven cyanobacterial strains from two reservoirs in São Paulo State, Brazil. Seven different chemical variants of microcystins (MC-RR, MC-LR, MC-YR, MC-LF, MC-LW, and two demethylated variants, dm-MC-RR and dm-MC-LR) were detected in three of the ten isolated strains. One particular Microcystis aeruginosa strain (LTPNA 02) was chosen to evaluate its growth by cell count, and its toxin production under seven different nutritional regimes. We observed different growth behaviors in the logarithmic growth period for only three experiments (p < 0.05). The total growth analysis identified four experiments as different from the control (p < 0.01). Three microcystin variants (MC-RR, MC-LR and MC-YR) were quantified by liquid chromatography-tandem mass spectrometry. At the experimental end, the toxin content was unchanged when comparing cell growth in ASM-1 (N:P = 1), MLA and BG-11 (N:P = 10) medium. In all other experiments, the lowest microcystin production was observed from cells grown in Bold 3N medium during the exponential growth phase. The highest microcystin content was observed in cultures using BG-11(N:P = 100) medium.en
dc.description.affiliationUniversidade de São Paulo Departamento de Análises Clínicas e Toxicológicas
dc.description.affiliationSaarland University Institute of Bioanalytical Chemistry
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Instituto de Ciências Ambientais, Químicas e Farmacêuticas
dc.description.affiliationFriedrich-Schiller University of Jena Institute of Nutrition
dc.description.affiliationHans Knöll Institute Leibniz Institute for Natural Product Research and Infection Biology
dc.description.affiliationUniversidade de São Paulo Instituto de Química Departamento de Bioquímica
dc.description.affiliationUnifespUNIFESP, Instituto de Ciências Ambientais, Químicas e Farmacêuticas
dc.description.provenanceMade available in DSpace on 2015-06-14T13:47:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-08-01. Added 1 bitstream(s) on 2015-08-11T04:00:31Z : No. of bitstreams: 1 S0102-695X2014000400389.pdf: 1073879 bytes, checksum: f387bbc649d2ad0d6219f89b01653506 (MD5)en
dc.description.sourceSciELO
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipAlfried Krupp von Bohlen und Halbach-Stiftung
dc.description.sponsorshipIDCNPq: 201609/2012-6
dc.format.extent389-398
dc.identifierhttp://dx.doi.org/10.1016/j.bjp.2014.07.019
dc.identifier.citationRevista Brasileira de Farmacognosia. Sociedade Brasileira de Farmacognosia, v. 24, n. 4, p. 389-398, 2014.
dc.identifier.doi10.1016/j.bjp.2014.07.019
dc.identifier.fileS0102-695X2014000400389.pdf
dc.identifier.issn0102-695X
dc.identifier.scieloS0102-695X2014000400389
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/8503
dc.identifier.wosWOS:000344329500004
dc.language.isoeng
dc.publisherSociedade Brasileira de Farmacognosia
dc.relation.ispartofRevista Brasileira de Farmacognosia
dc.rightsAcesso aberto
dc.subjectCyanobacteriaen
dc.subjectMicrocystis sp.en
dc.subjectMicrocystinen
dc.subjectLC-MS/MSen
dc.titleGrowth and microcystin production of a Brazilian Microcystis aeruginosa strain (LTPNA 02) under different nutrient conditionsen
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