Effect of Collagen Cross-linking in Stromal Fibril Organization in Edematous Human Corneas

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dc.contributor.authorBottos, Katia Mantovani [UNIFESP]
dc.contributor.authorHofling-Lima, Ana Luisa [UNIFESP]
dc.contributor.authorBarbosa, Manuela C. [UNIFESP]
dc.contributor.authorBarbosa, Jose Bonifacio [UNIFESP]
dc.contributor.authorDreyfuss, Juliana L. [UNIFESP]
dc.contributor.authorSchor, Paulo [UNIFESP]
dc.contributor.authorNader, Helena B. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:05:09Z
dc.date.available2016-01-24T14:05:09Z
dc.date.issued2010-07-01
dc.description.abstractPurpose: To assess structural stromal modifications after riboflavin and UV-A exposure in edematous human corneas.Method: Fourteen eyes with corneal edema were enrolled in the study. in the cross-linking (CXL) group, 7 corneal buttons were obtained from 6 patients who underwent penetrating keratoplasty (PK) 7-90 days after the CXL treatment. the control group was composed of 7 corneal buttons with bullous keratopathy. After the PK, stromal modifications were investigated using immunofluorescence in all corneal grafts. All patients had at least 3 months of corneal edema and were in the eye bank list waiting for keratoplasty.Results: All corneas in the treated group showed a pronounced lamellar zone of collagen fibers highly organized in the anterior stroma, but there was not complete homogeneity between the samples. Corneas with advanced disease and stromal fibrosis were less compacted than ones with mild disease severity. Similarly, those ones that underwent PK 3 months after CXL also showed a decreased effect compared with those with a reduced time between the CXL and the PK. DAPI staining demonstrated a complete fragmentation of keratocytes nuclei in the anterior stroma in all treated corneas, which were absent in the control group.Conclusions: Our study showed an immediate effect of CXL with a limited long-term sustainability. Cross-linked corneas had a pronounced anterior zone of organized collagen fibers. Even the treated corneas with advanced bullous keratopathy and stromal fibrosis had histological evidence of collagen fibers organization, but this effect seems to be decreased compared with corneas in initial stages of the disease.en
dc.description.affiliationUniversidade Federal de São Paulo, Paulista Sch Med, Dept Ophthalmol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Paulista Sch Med, Div Mol Biol, Dept Biochem, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Paulista Sch Med, Dept Ophthalmol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Paulista Sch Med, Div Mol Biol, Dept Biochem, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipVision Institute from UNIFESP Ophthalmology Department
dc.format.extent789-793
dc.identifierhttp://dx.doi.org/10.1097/ICO.0b013e3181bd9f12
dc.identifier.citationCornea. Philadelphia: Lippincott Williams & Wilkins, v. 29, n. 7, p. 789-793, 2010.
dc.identifier.doi10.1097/ICO.0b013e3181bd9f12
dc.identifier.issn0277-3740
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32696
dc.identifier.wosWOS:000280253300013
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofCornea
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectcross-linkingen
dc.subjectriboflavinen
dc.subjectedematous corneasen
dc.subjectUV-Aen
dc.subjectCXLen
dc.subjectimmunofluorescenceen
dc.subjectbullous keratopathyen
dc.titleEffect of Collagen Cross-linking in Stromal Fibril Organization in Edematous Human Corneasen
dc.typeinfo:eu-repo/semantics/article
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