Tracing Genetic Exchange and Biogeography of Cryptococcus neoformans var. grubii at the Global Population Level

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dc.contributor.authorRhodes, Johanna
dc.contributor.authorDesjardins, Christopher A.
dc.contributor.authorSykes, Sean M.
dc.contributor.authorBeale, Mathew A.
dc.contributor.authorVanhove, Mathieu
dc.contributor.authorSakthikumar, Sharadha
dc.contributor.authorChen, Yuan
dc.contributor.authorGujja, Sharvari
dc.contributor.authorSaif, Sakina
dc.contributor.authorChowdhary, Anuradha
dc.contributor.authorLawson, Daniel John
dc.contributor.authorPonzio, Vinicius [UNIFESP]
dc.contributor.authorColombo, Arnaldo Lopes [UNIFESP]
dc.contributor.authorMeyer, Wieland
dc.contributor.authorEngelthaler, David M.
dc.contributor.authorHagen, Ferry
dc.contributor.authorTeresa Illnait-Zaragozi, Maria
dc.contributor.authorAlanio, Alexandre
dc.contributor.authorVreulink, Jo-Marie
dc.contributor.authorHeitman, Joseph
dc.contributor.authorPerfect, John R.
dc.contributor.authorLitvintseva, Anastasia P.
dc.contributor.authorBicanic, Tihana
dc.contributor.authorHarrison, Thomas S.
dc.contributor.authorFisher, Matthew C.
dc.contributor.authorCuomo, Christina A.
dc.date.accessioned2019-08-19T11:48:42Z
dc.date.available2019-08-19T11:48:42Z
dc.date.issued2017
dc.description.abstractCryptococcus neoformans var. grubii is the causative agent of cryptococcal meningitis, a significant source of mortality in immunocompromised individuals, typically human immunodeficiency virus/AIDS patients from developing countries. Despite the worldwide emergence of this ubiquitous infection, little is known about the global molecular epidemiology of this fungal pathogen. Here we sequence the genomes of 188 diverse isolates and characterize the major subdivisions, their relative diversity, and the level of genetic exchange between them. While most isolates of C. neoformans var. grubii belong to one of three major lineages (VNI, VNII, and VNB), some haploid isolates show hybrid ancestry including some that appear to have recently interbred, based on the detection of large blocks of each ancestry across each chromosome. Many isolates display evidence of aneuploidy, which was detected for all chromosomes. In diploid isolates of C. neoformans var. grubii (serotype AA) and of hybrids with C. neoformans var. neoformans (serotype AD) such aneuploidies have resulted in loss of heterozygosity, where a chromosomal region is represented by the genotype of only one parental isolate. Phylogenetic and population genomic analyses of isolates from Brazil reveal that the previously "African" VNB lineage occurs naturally in the South American environment. This suggests migration of the VNB lineage between Africa and South America prior to its diversification, supported by finding ancestral recombination events between isolates from different lineages and regions. The results provide evidence of substantial population structure, with all lineages showing multi-continental distributionsen
dc.description.abstractdemonstrating the highly dispersive nature of this pathogen.en
dc.description.affiliationImperial Coll London, Dept Infect Dis Epidemiol, London W2 1PG, England
dc.description.affiliationMassachusetts Inst Technol & Harvard, Broad Inst, Infect Dis & Microbiome Program, Cambridge, MA 02142 USA
dc.description.affiliationSt Georges Univ London, Inst Infect & Immun, London WC1E 6BT, England
dc.description.affiliationWellcome Trust Sanger Inst, Infect Genom, Wellcome Genome Campus, Cambridge CB10 1SA, England
dc.description.affiliationDuke Univ, Med Ctr, Dept Med, Div Infect Dis, Durham, NC 27710 USA
dc.description.affiliationDuke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA
dc.description.affiliationUniv Delhi, Vallabhbhai Patel Chest Inst, Dept Med Mycol, Delhi 110007, India
dc.description.affiliationUniv Bristol, Sch Social & Community Med, Integrat Epidemiol Unit, Bristol BS8 1TH, Avon, England
dc.description.affiliationUniv Fed São Paulo, Div Infect Dis, BR-04039032 São Paulo, Brazil
dc.description.affiliationUniv Sydney, Sydney Med Sch Westmead Hosp, Marie Bashir Inst Infect Dis & Biosecur, Westmead Inst Med Res,Mol Mycol Res Lab, Sydney, NSW 2145, Australia
dc.description.affiliationFundação Oswaldo Cruz, Evandro Chagas Natl Inst Infect Dis, Mycol Lab, BR-21040360 Rio De Janeiro, Brazil
dc.description.affiliationTGen North, Translat Genom Res Inst, Flagstaff, AZ 86005 USA
dc.description.affiliationCanisius Wilhelmina Hosp, Dept Med Microbiol & Infect Dis, NL-6532SZ Nijmegen, Netherlands
dc.description.affiliationRadboudumc Canisius Wilhelmina Hosp, Ctr Expertise Mycol, NL-6532SZ Nijmegen, Netherlands
dc.description.affiliationInst Medicina Trop Pedro Kouri, Dept Bacteriol Micol, Ctr Invest Diagnost & Referencia, Havana 601, Cuba
dc.description.affiliationGrp Hosp St Louis Lariboisiere Fernand Widal, Lab Parasitol Mycol, AP HP, F-75010 Paris, France
dc.description.affiliationUniv Paris Diderot, Sorbonne Paris Cite, F-75010 Paris, France
dc.description.affiliationCtr Natl Reference Mycoses Invas & Antifong, Ctr Natl Rech Sci, Inst Pasteur, Unite Mycol Mol,URA3012, F-75015 Paris, France
dc.description.affiliationStellenbosch Univ, Dept Microbiol, ZA-7600 Stellenbosch, South Africa
dc.description.affiliationUnifespUniv Fed São Paulo, Div Infect Dis, BR-04039032 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipNational Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health, Department of Health and Human Services
dc.description.sponsorshipNational Human Genome Research Institute (NHGRI)
dc.description.sponsorshipU.S. Public Health Service (USPHS)
dc.description.sponsorshipUnited Kingdom Medical Research Council (MRC)
dc.description.sponsorshipUnited Kingdom Natural Environment Research Council
dc.description.sponsorshipNational Institutes of Health (NIH)
dc.description.sponsorshipWellcome Trust (WT)
dc.description.sponsorshipIDNIAID: U19 AI-110818
dc.description.sponsorshipIDNHGRI: U54HG003067
dc.description.sponsorshipIDUSPHS: AI73896
dc.description.sponsorshipIDUSPHS: AI93257
dc.description.sponsorshipIDMRC: MR/K000373/1
dc.description.sponsorshipIDNIH: AI-39115-19
dc.description.sponsorshipIDNIH: AI-50113-13
dc.description.sponsorshipIDWT: 104125MA
dc.format.extent327-346
dc.identifierhttp://dx.doi.org/10.1534/genetics.117.203836
dc.identifier.citationGenetics. Bethesda, v. 207, n. 1, p. 327-346, 2017.
dc.identifier.doi10.1534/genetics.117.203836
dc.identifier.fileWOS000409179800025.pdf
dc.identifier.issn0016-6731
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/51325
dc.identifier.wosWOS:000409179800025
dc.language.isoeng
dc.publisherGenetics Society America
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCryptococcusen
dc.subjecthybridizationen
dc.subjectphylogeographyen
dc.subjectrecombinationen
dc.subjectselectionen
dc.subjectgenome sequenceen
dc.titleTracing Genetic Exchange and Biogeography of Cryptococcus neoformans var. grubii at the Global Population Levelen
dc.typeinfo:eu-repo/semantics/article
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