Effect of the exposure to fine inhalable particulate matter (pm2.5) on sperm functional quality of mice

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dc.citation.issue3pt_BR
dc.citation.volume114pt_BR
dc.contributor.authorIntasqui, Paula
dc.contributor.authorTamashiro, Letícia Kaory
dc.contributor.authorYariwake, Victor Yuji
dc.contributor.authorSouza, Rosana Xavier
dc.contributor.authorKanashiro, Camila Matie
dc.contributor.authorVeras, Mariana Matera
dc.contributor.authorLatteshttp://lattes.cnpq.br/5188240479960852pt_BR
dc.date.accessioned2021-01-15T11:54:53Z
dc.date.available2021-01-15T11:54:53Z
dc.date.issued2020-09-01
dc.description.abstractObjective: To evaluate the effect of exposure to pollution (fine inhalable particulate matter - PM2.5) from the city of São Paulo on sperm functional quality. Design: Male isogenic BALB/c mice were used, distributed in two groups, control (n=6) and polluted air (n=8). For the polluted air group, after weaning (21 days), animals were daily exposed to 600 μg/m3 of PM2,5 for 96 days in an Ambient Particle Concentrator (APC). Control group was simultaneously exposed to filtered air in the APC. On postnatal day 118, animals were sacrificed (isoflurane overdose), body was weighted and the epididymis were collected. Materials and Methods: Sperm obtained from the cauda epididymis were used for the evaluation of motility, mitochondrial activity (DAB staining), acrosome integrity (PNA staining), DNA fragmentation (alkaline comet assay), oxidative stress (DHE staining) and cell viability (PI staining). Groups were compared using an unpaired Student’s t test (p<0.05). Results: Groups did not differ regarding body weight, and sperm motility. Furthermore, air pollution did not alter sperm functional quality (Table 1). Conclusions: Exposure to high concentrations of PM2.5 does not affect sperm motility and functional parameters.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extentE17-E18pt_BR
dc.identifierhttps://www.fertstert.org/article/S0015-0282(20)30828-1/fulltextpt_BR
dc.identifier.doihttps://doi.org/10.1016/j.fertnstert.2020.08.074pt_BR
dc.identifier.issn0015-0282pt_BR
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/59038
dc.languageengpt_BR
dc.publisherAmerican Society for Reproductive Medicinept_BR
dc.relation.ispartofFertility and Sterilitypt_BR
dc.rightsAcesso abertopt_BR
dc.subjectAir pollutionpt_BR
dc.subjectSpermatozoapt_BR
dc.subjectDNA Fragmentationpt_BR
dc.subjectOxidative Stresspt_BR
dc.titleEffect of the exposure to fine inhalable particulate matter (pm2.5) on sperm functional quality of micept_BR
dc.typeTrabalho apresentado em eventopt_BR
unifesp.campusEscola Paulista de Medicina (EPM)pt_BR
unifesp.departamentoCirurgiapt_BR
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