Reflection imaging of China ink-perfused brain vasculature using confocal laser-scanning microscopy after clarification of brain tissue by the Spalteholz method

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dc.citation.issue4
dc.citation.volume230
dc.contributor.authorGutierre, R. C. [UNIFESP]
dc.contributor.authorVannucci Campos, D. [UNIFESP
dc.contributor.authorMortara, R. A. [UNIFESP
dc.contributor.authorCoppi, A. A.
dc.contributor.authorArida, R. M. [UNIFESP
dc.coverageHoboken
dc.date.accessioned2020-07-17T14:02:34Z
dc.date.available2020-07-17T14:02:34Z
dc.date.issued2017
dc.description.abstractConfocal laser- scanning microscopy is a useful tool for visualizing neurons and glia in transparent preparations of brain tissue from laboratory animals. Currently, imaging capillaries and venules in transparent brain tissues requires the use of fluorescent proteins. Here, we show that vessels can be imaged by confocal laser- scanning microscopy in transparent cortical, hippocampal and cerebellar preparations after clarification of China inkinjected specimens by the Spalteholz method. This method may be suitable for global, three- dimensional, quantitative analyses of vessels, including stereological estimations of total volume and length and of surface area of vessels, which constitute indirect approaches to investigate angiogenesis.en
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Neurol & Neurosurg, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUniv Surrey, Fac Hlth & Med Sci, Sch Vet Med, Guildford, Surrey, England
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Physiol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Neurol & Neurosurg, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Physiol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCAPES PNPD
dc.description.sponsorshipFAPESP
dc.description.sponsorshipCNPq
dc.description.sponsorshipInstituto Nacional de NeurocienciaTranslacional (INNT)
dc.format.extent601-606
dc.identifierhttp://dx.doi.org/10.1111/joa.12578
dc.identifier.citationJournal Of Anatomy. Hoboken, v. 230, n. 4, p. 601-606, 2017.
dc.identifier.doi10.1111/joa.12578
dc.identifier.issn0021-8782
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54867
dc.identifier.wosWOS:000398042300011
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofJournal Of Anatomy
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectbrainen
dc.subjectclarificationen
dc.subjectconfocal microscopyen
dc.subjectstereologyen
dc.subjectvesselsen
dc.titleReflection imaging of China ink-perfused brain vasculature using confocal laser-scanning microscopy after clarification of brain tissue by the Spalteholz methoden
dc.typeinfo:eu-repo/semantics/article
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