Effects of two different decellularization routes on the mechanical properties of decellularized lungs

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dc.citation.issue6
dc.citation.volume12
dc.contributor.authorUrbano, Jessica Julioti
dc.contributor.authorda Palma, Renata Kelly
dc.contributor.authorde Lima, Flavia Mafra [UNIFESP]
dc.contributor.authorFratini, Paula
dc.contributor.authorGuimaraes, Leticia Lopes
dc.contributor.authorUriarte, Juan J.
dc.contributor.authorAlvarenga, Leticia Heineck
dc.contributor.authorMiglino, Maria Angelica
dc.contributor.authorVieira, Rodolfo de Paula
dc.contributor.authorPrates, Renato Araujo
dc.contributor.authorNavajas, Daniel
dc.contributor.authorFarre, Ramon
dc.contributor.authorFranco Oliveira, Luis Vicente
dc.coverageSan Francisco
dc.date.accessioned2020-07-13T11:53:02Z
dc.date.available2020-07-13T11:53:02Z
dc.date.issued2017
dc.description.abstractConsidering the limited number of available lung donors, lung bioengineering using whole lung scaffolds has been proposed as an alternative approach to obtain lungs suitable for transplantation. However, some decellularization protocols can cause alterations on the structure, composition, or mechanical properties of the lung extracellular matrix. Therefore, the aim of this study was to compare the acellular lung mechanical properties when using two different routes through the trachea and pulmonary artery for the decellularization process. This study was performed by using the lungs excised from 30 healthy male C57BL/6 mice, which were divided into 3 groups: tracheal decellularization (TDG), perfusion decellularization (PDG), and control groups (CG). Both decellularized groups were subjected to decellularization protocol with a solution of 1% sodium dodecyl sulfate. The behaviour of mechanical properties of the acellular lungs was measured after decellularization process. Static (Est) and dynamic (Edyn) elastances were obtained by the end-inspiratory occlusion method. TDG and PDG showed reduced Est and Edyn elastances after lung decellularization. Scanning electron microscopy showed no structural changes after lung decellularization of the TDG and PDG. In conclusion, was demonstrated that there is no significant difference in the behaviour of mechanical properties and extracellular matrix of the decellularized lungs by using two different routes through the trachea and pulmonary artery.en
dc.description.affiliationNove De Julho Univ, Rehabil Sci, Expt Cardioresp Physiol Lab, Sao Paulo, Brazil
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Dept Med, Div Nephrol, Lab Clin & Expt Immunol, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Vet Med & Zootecny, Dept Surg, Sao Paulo, Brazil
dc.description.affiliationUniv Barcelona, IDIBAPS, Fac Med,IBEC, Unitat Biofis & Bioengn,CIBER Enfermedades Resp, Barcelona, Spain
dc.description.affiliationNove De Julho Univ, Biophoton Appl Hlth Sci, Sao Paulo, Brazil
dc.description.affiliationUnifespFed Univ Sao Paulo UNIFESP, Dept Med, Div Nephrol, Lab Clin & Expt Immunol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipMinisterio de Economia y Competitividad
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (acronym CNPq)
dc.description.sponsorshipResearch Productivity modality PQIB
dc.description.sponsorshipIDMinisterio de Economia y Competitividad: PI1400280
dc.description.sponsorshipIDMinisterio de Economia y Competitividad: PI14/0004
dc.description.sponsorshipIDPQIB: 311426/2014-0
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0178696
dc.identifier.citationPlos One. San Francisco, v. 12, n. 6, p. -, 2017.
dc.identifier.doi10.1371/journal.pone.0178696
dc.identifier.fileWOS000402611800105pdf
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54359
dc.identifier.wosWOS:000402611800105
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleEffects of two different decellularization routes on the mechanical properties of decellularized lungsen
dc.typeinfo:eu-repo/semantics/article
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