Microbial community structure and dynamics in thermophilic composting viewed through metagenomics and metatranscriptomics

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dc.citation.volume6
dc.contributor.authorAntunes, Luciana Principal
dc.contributor.authorMartins, Layla Farage
dc.contributor.authorPereira, Roberta Verciano
dc.contributor.authorThomas, Andrew Maltez
dc.contributor.authorBarbosa, Deibs
dc.contributor.authorLemos, Leandro Nascimento
dc.contributor.authorMachado Silva, Gianluca Major
dc.contributor.authorSilva Moura, Livia Maria
dc.contributor.authorCondomitti Epamino, George Willian
dc.contributor.authorDigiampietri, Luciano Antonio
dc.contributor.authorLombardi, Karen Cristina
dc.contributor.authorRamos, Patricia Locosque
dc.contributor.authorQuaggio, Ronaldo Bento
dc.contributor.authorOliveira, Julio Cezar Franco de [UNIFESP]
dc.contributor.authorPascon, Renata Castiglioni [UNIFESP]
dc.contributor.authorda Cruz, Joao Batista
dc.contributor.authorda Silva, Aline Maria
dc.contributor.authorSetubal, Joao Carlos
dc.coverageLondon
dc.date.accessioned2020-07-31T12:47:03Z
dc.date.available2020-07-31T12:47:03Z
dc.date.issued2016
dc.description.abstractComposting is a promising source of new organisms and thermostable enzymes that may be helpful in environmental management and industrial processes. Here we present results of metagenomicand metatranscriptomic-based analyses of a large composting operation in the Sao Paulo Zoo Park. This composting exhibits a sustained thermophilic profile (50 degrees C to 75 degrees C), which seems to preclude fungal activity. The main novelty of our study is the combination of time-series sampling with shotgun DNA, 16S rRNA gene amplicon, and metatranscriptome high-throughput sequencing, enabling an unprecedented detailed view of microbial community structure, dynamics, and function in this ecosystem. The time-series data showed that the turning procedure has a strong impact on the compost microbiota, restoring to a certain extent the population profile seen at the beginning of the processen
dc.description.abstractand that lignocellulosic biomass deconstruction occurs synergistically and sequentially, with hemicellulose being degraded preferentially to cellulose and lignin. Moreover, our sequencing data allowed near-complete genome reconstruction of five bacterial species previously found in biomass-degrading environments and of a novel biodegrading bacterial species, likely a new genus in the order Bacillales. The data and analyses provided are a rich source for additional investigations of thermophilic composting microbiology.en
dc.description.affiliationUniv Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Programa Pos Graduacao Interunidades Bioinformat, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Escola Artes Ciencias & Humanidades, Sao Paulo, Brazil|Fundacao Parque Zool Sao Paulo, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Ciencias Biol, Sao Paulo, Brazil
dc.description.affiliationBiocomplex Inst Virginia, Blacksburg, VA USA
dc.description.affiliationUnifespDepartamento de Ciências Biológicas, Universidade Federal de São Paulo, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipProvost's Office for Research of the University of Sao Paulo
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2011/50870-6
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1038/srep38915
dc.identifier.citationScientific Reports. London, v. 6, p. -, 2016.
dc.identifier.doi10.1038/srep38915
dc.identifier.fileWOS000389691100001.pdf
dc.identifier.issn2045-2322
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56552
dc.identifier.wosWOS:000389691100001
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofScientific Reports
dc.rightsAcesso aberto
dc.titleMicrobial community structure and dynamics in thermophilic composting viewed through metagenomics and metatranscriptomicsen
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