CD8(+)-T-cell-dependent control of Trypanosoma cruzi infection in a highly susceptible mouse strain after immunization with recombinant proteins based on amastigote surface protein 2

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dc.contributor.authorAraujo, Adriano Fernando da Silva [UNIFESP]
dc.contributor.authorAlencar, Bruna Cunha Gondim de [UNIFESP]
dc.contributor.authorVasconcelos, Jose Ronnie Carvalho de [UNIFESP]
dc.contributor.authorHiyane, Meire Ioshie [UNIFESP]
dc.contributor.authorMarinho, Claudio Romero Farias
dc.contributor.authorPenido, Marcus Luiz de Oliveira
dc.contributor.authorBoscardin, Silvia Beatriz [UNIFESP]
dc.contributor.authorHoft, Daniel F.
dc.contributor.authorGazzinelli, Ricardo Tostes
dc.contributor.authorRodrigues, Mauricio Martins [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionSt Louis Univ
dc.date.accessioned2016-01-24T12:38:01Z
dc.date.available2016-01-24T12:38:01Z
dc.date.issued2005-09-01
dc.description.abstractWe previously described that DNA vaccination with the gene encoding amastigote surface protein 2 (ASP-2) protects approximately 65% of highly susceptible A/Sn mice against the lethal Trypanosoma cruzi infection. Here, we explored the possibility that bacterial recombinant proteins of ASP-2 could be used to improve the efficacy of vaccinations. Initially, we compared the protective efficacy of vaccination regimens using either a plasmid DNA, a recombinant protein, or both sequentially (DNA priming and protein boosting). Survival after the challenge was not statistically different among the three mouse groups and ranged from 53.5 to 75%. the fact that immunization with a recombinant protein alone induced protective immunity revealed the possibility that this strategy could be pursued for vaccination. We investigated this possibility by using six different recombinant proteins representing distinct portions of ASP-2. the vaccination of mice with glutathione S-transferase fusion proteins representing amino acids 261 to 500 or 261 to 380 of ASP-2 in the presence of the adjuvants alum and CpG oligodeoxynucleotide 1826 provided remarkable immunity, consistently protecting 100% of the A/Sn mice. Immunity was completely reversed by the in vivo depletion of CD8(+) T cells, but not CD4(+) T cells, and was associated with the presence of CD8(+) T cells specific for an epitope located between amino acids 320 and 327 of ASP-2. We concluded that a relatively simple formulation consisting of a recombinant protein with a selected portion of ASP-2, alum, and CpG oligodeoxynucleotide 1826 might be used to cross-prime strong CD8(+)-T-cell-dependent protective immunity against T. cruzi infection.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, Dept Imunol,ICB, BR-05508900 São Paulo, Brazil
dc.description.affiliationUNIFESP, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Dept Bioquim & Imunol, Inst Ciencias Biol, BR-30190002 Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Ctr Pesquisas Rene Rachou, FIOCRUZ, BR-30190002 Belo Horizonte, MG, Brazil
dc.description.affiliationSt Louis Univ, Hlth Sci Ctr, Dept Internal Med, Div Infect Dis & Immunol, St Louis, MO 63110 USA
dc.description.affiliationSt Louis Univ, Hlth Sci Ctr, Dept Mol Microbiol, Div Infect Dis & Immunol, St Louis, MO 63110 USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, Dept Imunol,ICB, BR-05508900 São Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, BR-05508900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent6017-6025
dc.identifierhttps://dx.doi.org/10.1128/IAI.73.9.6017-6025.2005
dc.identifier.citationInfection and Immunity. Washington: Amer Soc Microbiology, v. 73, n. 9, p. 6017-6025, 2005.
dc.identifier.doi10.1128/IAI.73.9.6017-6025.2005
dc.identifier.fileWOS000231460000079.pdf
dc.identifier.issn0019-9567
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/28434
dc.identifier.wosWOS:000231460000079
dc.language.isoeng
dc.publisherAmer Soc Microbiology
dc.relation.ispartofInfection and Immunity
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleCD8(+)-T-cell-dependent control of Trypanosoma cruzi infection in a highly susceptible mouse strain after immunization with recombinant proteins based on amastigote surface protein 2en
dc.typeinfo:eu-repo/semantics/article
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