Crotamine induces browning of adipose tissue and increases energy expenditure in mice

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dc.citation.volumev. 8
dc.contributor.authorMarinovic, Marcelo Paradiso [UNIFESP]
dc.contributor.authorCampeiro, Joana Darc [UNIFESP]
dc.contributor.authorLima, Sunamita C. [UNIFESP]
dc.contributor.authorRocha, Andrea L. [UNIFESP]
dc.contributor.authorNering, Marcela B. [UNIFESP]
dc.contributor.authorOliveira, Eduardo B.
dc.contributor.authorMori, Marcelo Alves da Silva [UNIFESP]
dc.contributor.authorHayashi, Mirian Akemi Furuie [UNIFESP]
dc.coverageLondon
dc.date.accessioned2020-07-20T16:31:14Z
dc.date.available2020-07-20T16:31:14Z
dc.date.issued2018
dc.description.abstractCrotamine, originally isolated from rattlesnake venom, has been extensively studied due to its pleiotropic biological properties, and special attention has been paid to its antitumor activity. However, long-term treatment with crotamine was accompanied by a reduction in animal body weight gain and by increases in glucose tolerance. As cancer is commonly associated with cachexia, to preclude the possible cancer cachexia-like effect of crotamine, herein this polypeptide was administered in healthy wild-type C57/BL6 mice by the oral route daily, for 21 days. Reduced body weight gain, in addition to decreased white adipose tissue (WAT) and increased brown adipose tissue (BAT) mass were observed in healthy animals in the absence of tumor. In addition, we observed improved glucose tolerance and increased insulin sensitivity, accompanied by a reduction of plasma lipid levels and decreased levels of biomarkers of liver damage and kidney disfunctions. Importantly, long-term treatment with crotamine increased the basal metabolic rate in vivo, which was consistent with the increased expression of thermogenic markers in BAT and WAT. Interestingly, cultured brown adipocyte cells induced to differentiation in the presence of crotamine also showed increases in some of these markers and in lipid droplets number and size, indicating increased brown adipocyte maturation.en
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, EPM, Dept Farmacol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, UNIFESP, EPM, Dept Biofis, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Bioquim & Imunol, Ribeirao Preto, Brazil
dc.description.affiliationUniv Estadual Campinas UNICAMP, Dept Bioquim & Biol Tecidual, Campinas, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, EPM, Dept Farmacol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, UNIFESP, EPM, Dept Biofis, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSao Paulo Research Foundation (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP)
dc.description.sponsorshipNational Council of Technological and Scientific Development (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq)
dc.description.sponsorshipIDFAPESP: 2013/13392-4
dc.description.sponsorshipIDFAPESP: 2017/02413-1
dc.description.sponsorshipIDCNPq: 311815/2012-0
dc.description.sponsorshipIDCNPq: 475739/2013-2
dc.description.sponsorshipIDCNPq: 39337/2016-0
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1038/s41598-018-22988-1
dc.identifier.citationScientific Reports. London, v. 8, 2018.
dc.identifier.doi10.1038/s41598-018-22988-1
dc.identifier.fileWOS000428034400008.pdf
dc.identifier.issn2045-2322
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55805
dc.identifier.wosWOS:000428034400008
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofScientific Reports
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleCrotamine induces browning of adipose tissue and increases energy expenditure in miceen
dc.typeinfo:eu-repo/semantics/article
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