Mg-Al and Zn-Al Layered Double Hydroxides Promote Dynamic Expression of Marker Genes in Osteogenic Differentiation by Modulating Mitogen-Activated Protein Kinases

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dc.citation.issue4
dc.citation.volume7
dc.contributor.authorKang, Ha Ram
dc.contributor.authorda Costa Fernandes, Celio Junior
dc.contributor.authorda Silva, Rodrigo Augusto
dc.contributor.authorLeopoldo Constantino, Vera Regina
dc.contributor.authorJun Koh, Ivan Hong [UNIFESP]
dc.contributor.authorZambuzzi, Willian F.
dc.coverageHoboken
dc.date.accessioned2020-07-08T13:09:39Z
dc.date.available2020-07-08T13:09:39Z
dc.date.issued2018
dc.description.abstractThe effect of LDH samples comprised of chloride anions intercalated between positive layers of magnesium/aluminum (Mg-Al LDH) or zinc/aluminum (Zn-Al LDH) chemical composition on pre-osteoblast performance is investigated. Non-cytotoxic concentrations of both LDHs modulated pre-osteoblast adhesion by triggering cytoskeleton rearrangement dependent on recruiting of Cofilin, which is modulated by the inhibition of the Protein Phosphatase 2A (PP2A), culminating in osteoblast differentiation with a significant increase of osteogenic marker genes. The alkaline phosphatase (ALP) and bone sialoprotein (BSP) are significantly up-modulated by both LDHsen
dc.description.abstracthowever, Mg-Al LDH nanomaterial promoted even more significance than both experimental controls, while the phosphorylations of mitogen-activated protein kinase (MAPKs)- extracellular signal-regulated kinases (ERK) and c-Jun N-terminal kinase (JNK) significantly increased. MAPK signaling is necessary to activate Runt-related transcription factor 2 (RUNX2) gene. Concomitantly, it is also investigated whether challenged osteoblasts are able to modulate osteoclastogenesis by investigating both osteoprotegerin (OPG) and Receptor activator of nuclear factor kappa-ligand (RANKL) in this modelen
dc.description.abstracta dynamic reprogramming of both these genes is found, suggesting LDHs in modulating osteoclastogenesis. These results suggest that LDHs interfere in bone remodeling, and they can be considered as nanomaterials in graft-based bone healing or drug-delivery materials for bone disorders.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Lab Bioensaios & Dinam Celular, Dept Quim & Bioquim,Inst Biociencias, Campus Botucatu, BR-18618970 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, USP, Inst Quim, Dept Quim Fundamental, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, UNIFESP, Dept Cirurgia, Rua Botucatu 740, BR-04023900 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, UNIFESP, Dept Cirurgia, Rua Botucatu 740, BR-04023900 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP
dc.description.sponsorshipCNPq
dc.description.sponsorshipIDFAPESP: 2014/22689-3, 2011/50318-1
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1002/adhm.201700693
dc.identifier.citationAdvanced Healthcare Materials. Hoboken, v. 7, n. 4, p. -, 2018.
dc.identifier.doi10.1002/adhm.201700693
dc.identifier.fileWOS000425622200002.pdf
dc.identifier.issn2192-2640
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54119
dc.identifier.wosWOS:000425622200002
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofAdvanced Healthcare Materials
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectdifferentiationen
dc.subjectdouble metal hydroxidesen
dc.subjecthydrotalcite-like materialsen
dc.subjectlayered double hydroxidesen
dc.subjectnanoparticlesen
dc.subjectosteoblastsen
dc.titleMg-Al and Zn-Al Layered Double Hydroxides Promote Dynamic Expression of Marker Genes in Osteogenic Differentiation by Modulating Mitogen-Activated Protein Kinasesen
dc.typeinfo:eu-repo/semantics/article
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