Identification of new-world leishmania using ribosomal gene spacer probes
| dc.contributor.author | Guevara, Palmira | |
| dc.contributor.author | Alonso, Guillermina | |
| dc.contributor.author | Silveira, Jose Franco da [UNIFESP] | |
| dc.contributor.author | Mello, Maricilda Palandi de [UNIFESP] | |
| dc.contributor.author | Scorza, José Vicente | |
| dc.contributor.author | Anez, Nestor | |
| dc.contributor.author | Ramirez, José Luis | |
| dc.contributor.institution | UNIV CENT VENEZUELA | |
| dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
| dc.contributor.institution | NUCL UNIV RAFAEL RANGEL | |
| dc.contributor.institution | UNIV LOS ANDES | |
| dc.date.accessioned | 2016-01-24T11:40:10Z | |
| dc.date.available | 2016-01-24T11:40:10Z | |
| dc.date.issued | 1992-11-01 | |
| dc.description.abstract | DNA probes from the nontranscribed ribosomal spacer (NTS), of Leishmania garnhami and Leishmania braziliensis were constructed and tested for sensitivity and specificity against different Leishmania isolates. the L. garnhami probes were species-specific under hybridization conditions of high stringency, but displayed specificity for the mexicana complex under conditions of intermediate stringency. the L. braziliensis probes showed 'complex' specificity. RFLP for the nontranscribed spacer within the braziliensis complex revealed very homogeneous patterns even for organisms currently accepted as different species. A PCR assay for the detection of Leishmania from the braziliensis complex is presented. | en |
| dc.description.affiliation | UNIV CENT VENEZUELA,CTR BIOL CELULAR,APARTADO 47525,CARACAS 1041A,VENEZUELA | |
| dc.description.affiliation | ESCOLA PAULISTA MED SCH,DEPT PARASITOL,BR-04023 São Paulo,BRAZIL | |
| dc.description.affiliation | NUCL UNIV RAFAEL RANGEL,TRUJILLO,VENEZUELA | |
| dc.description.affiliation | UNIV LOS ANDES,FAC CIENCIAS,MERIDA,VENEZUELA | |
| dc.description.affiliationUnifesp | ESCOLA PAULISTA MED SCH,DEPT PARASITOL,BR-04023 São Paulo,BRAZIL | |
| dc.description.source | Web of Science | |
| dc.format.extent | 15-26 | |
| dc.identifier | https://dx.doi.org/10.1016/0166-6851(92)90150-I | |
| dc.identifier.citation | Molecular and Biochemical Parasitology. Amsterdam: Elsevier B.V., v. 56, n. 1, p. 15-26, 1992. | |
| dc.identifier.doi | 10.1016/0166-6851(92)90150-I | |
| dc.identifier.issn | 0166-6851 | |
| dc.identifier.uri | https://repositorio.unifesp.br/handle/11600/25283 | |
| dc.identifier.wos | WOS:A1992KA72600002 | |
| dc.language.iso | eng | |
| dc.publisher | Elsevier B.V. | |
| dc.relation.ispartof | Molecular and Biochemical Parasitology | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
| dc.subject | Parasitic protozoan | en |
| dc.subject | Leishmania-identification | en |
| dc.subject | Ribosomal spacer | en |
| dc.subject | DNA probe | en |
| dc.subject | RFLP | en |
| dc.subject | PCR | en |
| dc.title | Identification of new-world leishmania using ribosomal gene spacer probes | en |
| dc.type | info:eu-repo/semantics/article |