Host cell invasion and oral infection by Trypanosoma cruzi strains of genetic groups TcI and TcIV from chagasic patients

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dc.citation.volume9
dc.contributor.authorMaeda, Fernando Yukio [UNIFESP]
dc.contributor.authorClemente, Tatiana Mordente [UNIFESP]
dc.contributor.authorMacedo, Silene [UNIFESP]
dc.contributor.authorCortez, Cristian [UNIFESP]
dc.contributor.authorYoshida, Nobuko [UNIFESP]
dc.coverageLondon
dc.date.accessioned2020-07-22T13:23:17Z
dc.date.available2020-07-22T13:23:17Z
dc.date.issued2016
dc.description.abstractBackground: Outbreaks of acute Chagas disease by oral infection have been reported frequently over the last ten years, with higher incidence in northern South America, where Trypanosoma cruzi lineage TcI predominates, being responsible for the major cause of resurgent human disease, and a small percentage is identified as TcIV. Mechanisms of oral infection and host-cell invasion by these parasites are poorly understood. To address that question, we analyzed T. cruzi strains isolated from chagasic patients in Venezuela, Guatemala and Brazil. Methods: Trypanosoma cruzi metacyclic trypomastigotes were orally inoculated into mice. The mouse stomach collected four days later, as well as the stomach and the heart collected 30 days post-infection, were processed for histological analysis. Assays to mimic parasite migration through the gastric mucus layer were performed by counting the parasites that traversed gastric mucin-coated transwell filters. For cell invasion assays, human epithelial HeLa cells were incubated with metacyclic forms and the number of internalized parasites was counted. Results: All TcI and TcIV T. cruzi strains were poorly infective by the oral route. Parasites were either undetectable or were detected in small numbers in the mouse stomach four days post oral administration. Replicating parasites were found in the stomach and/or in the heart 30 days post-infection. As compared to TcI lineage, the migration capacity of TcIV parasites through the gastric mucin-coated filter was higher but lower than that exhibited by TcVI metacyclic forms previously shown to be highly infective by the oral route. Expression of pepsin-resistant gp90, the surface molecule that downregulates cell invasion, was higher in TcI than in TcIV parasites and, accordingly, the invasion capacity of TcIV metacyclic forms was higher. Gp90 molecules spontaneously released by TcI metacyclic forms inhibited the parasite entry into host cells. TcI parasites exhibited low intracellular replication rate. Conclusions: Our findings indicate that the poor capacity of TcI lineage, and to a lesser degree of TcIV parasites, in invading gastric epithelium after oral infection of mice may be associated with the inefficiency of metacyclic forms, in particular of TcI parasites, to migrate through the gastric mucus layer, to invade target epithelial cells and to replicate intracellularly.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipIDFAPESP: 11/51475-3
dc.description.sponsorshipIDCNPq: 300578/2010-5
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1186/s13071-016-1455-z
dc.identifier.citationParasites & Vectors. London, v. 9, p. -, 2016.
dc.identifier.doi10.1186/s13071-016-1455-z
dc.identifier.fileWOS000373438700001.pdf
dc.identifier.issn1756-3305
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56154
dc.identifier.wosWOS:000373438700001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofParasites & Vectors
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTrypanosoma cruzien
dc.subjectTcI and TcIV lineagesen
dc.subjectMetacyclic trypomastigotesen
dc.subjectOral infectionen
dc.subjectHost cell invasionen
dc.titleHost cell invasion and oral infection by Trypanosoma cruzi strains of genetic groups TcI and TcIV from chagasic patientsen
dc.typeinfo:eu-repo/semantics/article
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