Expression of adhesion molecules on CD34+ cells from steady-state bone marrow before and after mobilization and their association with the yield of CD34+ cells

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dc.citation.issue1
dc.citation.volumev. 53
dc.contributor.authorCecyn, Karin Zattar [UNIFESP]
dc.contributor.authorKimura, Eliza Yuriko Sugano [UNIFESP]
dc.contributor.authorLima, Dulce Marta S. M. [UNIFESP]
dc.contributor.authorYamamoto, Miyoko [UNIFESP]
dc.contributor.authorBordin, Jose Orlando [UNIFESP]
dc.contributor.authorOliveira, José Salvador Rodrigues de [UNIFESP]
dc.coverageSeoul
dc.date.accessioned2020-07-20T16:31:18Z
dc.date.available2020-07-20T16:31:18Z
dc.date.issued2018
dc.description.abstractBackground Cell adhesion molecules (CAMs) expressed on hematopoietic progenitor cells (HPCs), endothelial cells, and stromal cells play a pivotal role in the mobilization of CD34+ cells. Herein, we conducted a non-randomized peripheral blood stem cell (PBSC) mobilization study aimed to compare the potential differences in the expressions of several CAMs and chemokines on CD34+ cells obtained from bone marrow aspirate before and after HPC mobilization from patients with hematologic malignancies and healthy donors. Methods Three-color cytofluorometric analysis was used to compare the expressions of CAMs and chemokines in the bone marrow before and after mobilization. Results For all studied groups, CAM expression among those with good and poor yields of CD34+ cells was significantly correlated with VCAM-1 (P=0.007), CD44 (P=0.027), and VLA-4 (P=0.014) expressions. VCAM-1 (P=0.001), FLT-3 (P=0.001), CD44 (P=0.011), VLA-4 (P=0.001), and LFA-1 (P=0,001) expressions were higher before HPC mobilization than after HPC mobilization. By contrast, the expression of CXCR4 significantly varied before and after mobilization only among those with successful PBSC mobilization (P=0.002). Conclusion We attempted to identify particular aspects of CAMs involved in CD34+ cell mobilization, which is a highly complex mechanism that involves adhesion molecules and matrix metalloproteases. The mechanism by which CD34+ cell mobilization is activated through proteolytic enzymes is not fully understood. We believe that CXCR4, VLA-4, CD44, and VCAM-1 are the most important molecules implicated in HPC mobilization, particularly because they show a correlation with the yield of CD34+ cells collected via large volume leukapheresis.en
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Oncol Clin & Expt, Rua Diogo de Faria 824, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Oncol Clin & Expt, Rua Diogo de Faria 824, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil
dc.format.extent61-70
dc.identifierhttp://dx.doi.org/10.5045/br.2018.53.1.61
dc.identifier.citationBlood Research. Seoul, v. 53, n. 1, p. 61-70, 2018.
dc.identifier.doi10.5045/br.2018.53.1.61
dc.identifier.fileWOS000429697000013.pdf
dc.identifier.issn2287-979X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55869
dc.identifier.wosWOS:000429697000013
dc.language.isoeng
dc.publisherKorean Soc Hematology
dc.relation.ispartofBlood Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAdhesion moleculesen
dc.subjectHematopoietic progenitor cellsen
dc.subjectMobilizationen
dc.subjectStem cell donoren
dc.subjectMultiple myelomaen
dc.subjectNon-Hodgkin lymphomaen
dc.titleExpression of adhesion molecules on CD34+ cells from steady-state bone marrow before and after mobilization and their association with the yield of CD34+ cellsen
dc.typeinfo:eu-repo/semantics/article
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