Purification, characterization, and cloning of a serine proteinase inhibitor from the ectoparasite Haematobia irritans irritans (Diptera : Muscidae)

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dc.contributor.authorAzzolini, S. S.
dc.contributor.authorSantos, JMC
dc.contributor.authorSouza, A. F.
dc.contributor.authorTorquato, RJS
dc.contributor.authorHirata, I. Y.
dc.contributor.authorAndreotti, R.
dc.contributor.authorTanaka, A. S.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2016-01-24T12:34:19Z
dc.date.available2016-01-24T12:34:19Z
dc.date.issued2004-03-01
dc.description.abstractThe fly Haematobia irritans irritant is one of the most important ectoparasites in cattle production, due to, its ability to suck large amounts of blood. This report describes the purification and characterization of a serine proteinase inhibitor (HiTI) present in H. i. irritans head and thorax extracts. the HiTI purified by, affinity chromatography on trypsin-Sepharose has a molecular mass of 7029 Da by MALDI-TOF mass spectrometry. HiTI inhibited bovine trypsin, human neutrophil elastase, and a trypsin-like enzyme, purified from H. i. irritans abdomen with dissociation constants of 0.57, 1.30, and 0.20 nM, respectively. the HiTI partial amino acid sequence allowed its classification into the BPTI-Kunitz-type family. An HiTI cDNA fragment was cloned in the pGEMT vector using RT-PCR. the translated amino acid sequence of HiTI cDNA confirmed a unique Kunitz-type-domain protein. Our results suggest that HiTI could control some endogenous enzyme, e.g., the H. i. irritans trypsin-like protein. (C) 2004 Elsevier Inc. All rights reserved.en
dc.description.affiliationEPM, UNIFESP, Dept Bioquim, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationEPM, UNIFESP, Dept Biofis, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationEmbrapa Gado Corte, Campo Grande, MS, Brazil
dc.description.affiliationUnifespEPM, UNIFESP, Dept Bioquim, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationUnifespEPM, UNIFESP, Dept Biofis, BR-04044020 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent103-109
dc.identifierhttp://dx.doi.org/10.1016/j.exppara.2004.01.012
dc.identifier.citationExperimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 106, n. 3-4, p. 103-109, 2004.
dc.identifier.doi10.1016/j.exppara.2004.01.012
dc.identifier.issn0014-4894
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27646
dc.identifier.wosWOS:000221992300005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofExperimental Parasitology
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectHaematobia irritans irritansen
dc.subjectSerine proteinase inhibitoren
dc.subjectKunitz-type inhibitoren
dc.subjectHiTIen
dc.subjectH. i. irritans trypsin inhibitoren
dc.subjectbovine trypsinen
dc.subjecthuman plasminen
dc.subjecthuman neutrophil elastaseen
dc.subjectbovine thrombinen
dc.subjecthuman factor Xaen
dc.subjecthuman factor XIIaen
dc.subjecthuman plasma kallikreinen
dc.subjectsubtilisin Aen
dc.subjectMALDI-TOFen
dc.subjectmatrix assisted laser desorption ionization time-of-flighten
dc.subjectcDNAen
dc.subjectcomplementary deoxyribonucleic aciden
dc.subjectRTPCRen
dc.subjectreverse transcription polymerase chain reactionen
dc.subjectTFAen
dc.subjecttrifluoroacetic aciden
dc.titlePurification, characterization, and cloning of a serine proteinase inhibitor from the ectoparasite Haematobia irritans irritans (Diptera : Muscidae)en
dc.typeArtigo
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