hTERT and TP53 deregulation in intestinal-type gastric carcinogenesis in non-human primates

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dc.contributor.authorLeal, Mariana Ferreira [UNIFESP]
dc.contributor.authorCalcagno, Danielle Queiroz [UNIFESP]
dc.contributor.authorKhayat, Andre Salim
dc.contributor.authorRaiol Silva, Tanielly Cristina
dc.contributor.authorPereira Carneiro Muniz, Jose Augusto
dc.contributor.authorAssumpcao, Paulo Pimentel
dc.contributor.authorCardoso Smith, Marilia de Arruda [UNIFESP]
dc.contributor.authorBurbano, Rommel Rodriguez
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFed Univ Para
dc.contributor.institutionMinist Saude
dc.date.accessioned2016-01-24T14:32:03Z
dc.date.available2016-01-24T14:32:03Z
dc.date.issued2013-08-01
dc.description.abstractDespite the high incidence, the molecular events involved in intestinal-type gastric carcinogenesis remains unclear. We previously established an intestinal-type gastric carcinogenesis model in Cebus apella, a New World monkey. in the present study, we evaluated hTERT and TP53 mRNA expression, as well as their protein immunoreactivity, in normal mucosa, non-atrophic gastritis, atrophic gastritis, intestinal metaplasia, and intestinal-type gastric cancer samples of non-human primates treated with N-methyl-nitrosourea. in addition, we evaluated the number of TP53 copies in these samples. Although hTERT immunoreactivity was only detected in gastric cancer, a continuous increase of hTERT mRNA expression was observed from non-atrophic gastritis to gastric tumors. No sample presented p53 immunoreactivity. However, we also observed a continuous decrease of TP53 mRNA expression during the sequential steps of gastric carcinogenesis. Moreover, loss of TP53 copies was observed in intestinal metaplasia and gastric cancer samples. Our study highlights that hTERT and TP53 have a key role in intestinal-type gastric cancer initiation.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Genet, BR-04023900 São Paulo, Brazil
dc.description.affiliationFed Univ Para, Inst Ciencias Biol, Lab Citogenet Humana, BR-66059 Belem, Para, Brazil
dc.description.affiliationMinist Saude, Ctr Nacl Primatas, Ananindeua, PA, Brazil
dc.description.affiliationFed Univ Para, Hosp Univ Joao de Barros Barreto, Unidade Alta Complexidade Oncol, BR-66059 Belem, Para, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Genet, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent221-224
dc.identifierhttp://dx.doi.org/10.1007/s10238-012-0195-4
dc.identifier.citationClinical and Experimental Medicine. Milan: Springer-verlag Italia Srl, v. 13, n. 3, p. 221-224, 2013.
dc.identifier.doi10.1007/s10238-012-0195-4
dc.identifier.issn1591-8890
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36584
dc.identifier.wosWOS:000322677400009
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofClinical and Experimental Medicine
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjecthTERTen
dc.subjectTP53en
dc.subjectGastric carcinogenesisen
dc.subjectNon-human primatesen
dc.subjectPrecancerous lesionsen
dc.subjectAnimal modelen
dc.titlehTERT and TP53 deregulation in intestinal-type gastric carcinogenesis in non-human primatesen
dc.typeinfo:eu-repo/semantics/article
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