SAGE analysis highlights the importance of p53csv, ddx5, mapkapk2 and ranbp2 to multiple myeloma tumorigenesis

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dc.contributor.authorFelix, Roberta S. [UNIFESP]
dc.contributor.authorColleoni, Gisele W. B. [UNIFESP]
dc.contributor.authorCaballero, Otavia L.
dc.contributor.authorYamamoto, Mihoko [UNIFESP]
dc.contributor.authorAlmeida, Manuella S. S. [UNIFESP]
dc.contributor.authorAndrade, Valeria C. C. [UNIFESP]
dc.contributor.authorChauffaille, Maria de Lourdes L. F. [UNIFESP]
dc.contributor.authorSilva, Wilson A. da
dc.contributor.authorBegnami, Maria Dirlei
dc.contributor.authorSoares, Fernando Augusto
dc.contributor.authorSimpson, Andrew J.
dc.contributor.authorZago, Marco Antonio
dc.contributor.authorVettore, Andre L. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionLudwig Inst Canc Res
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionFundacao Antonio Prudente
dc.date.accessioned2016-01-24T13:52:38Z
dc.date.available2016-01-24T13:52:38Z
dc.date.issued2009-06-08
dc.description.abstractSerial analysis of gene expression (SAGE) allows a comprehensive profiling of gene expression within a given tissue and also an assessment of transcript abundance. We generated SAGE libraries from normal and neoplastic plasma cells to identify genes differentially expressed in multiple myeloma (MM). Normal plasma cells were obtained from palatine tonsils and MM SAGE library was generated from bone marrow plasma cells of MM patients. We obtained 29,918 SAGE tags from normal and 10,340 tags from tumor libraries. Computer-gene rated genomic analysis identified 46 upregulated genes in the MM library. Ten upregulated genes were selected for further investigation. Differential expression was validated by quantitative real-time PCR in purified plasma cells of 31 patients and three controls. P53CSV, DDX5, MAPKAPK2 and RANBP2 were found to be upregulated in at least 50% of the MM cases tested. All of them were also found upregulated in MM when compared to normal plasma cells in a meta-analysis using ONCOMINE microarray database. Antibodies specific to DDX5, RANBP2 and MAPKAPK2 were used in a TMA containing 57 MM cases and confirmed the expression of these proteins in 74%, 96%, and 21% of the MM samples, respectively. Analysis of differential expression using SAGE could identify genes important for myeloma tumorigenesis (P53CSV, DDX5, MAPKPK2 and RANBP2) and that could potentially be useful as therapeutic targets. (c) 2008 Elsevier Ireland Ltd. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Hematol & Hemotherapy Serv, UNIFESP EPM, BR-04023900 São Paulo, Brazil
dc.description.affiliationLudwig Inst Canc Res, New York Branch, New York, NY USA
dc.description.affiliationUSP, Fac Med Ribeirao Preto, Ribeirao Preto, Brazil
dc.description.affiliationFundacao Antonio Prudente, Hosp AC Camargo, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biol Sci, UNIFESP EPM, BR-04023900 São Paulo, Brazil
dc.description.affiliationLudwig Inst Canc Res, São Paulo Branch, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Hematol & Hemotherapy Serv, UNIFESP EPM, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biol Sci, UNIFESP EPM, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 04/13213-3
dc.description.sponsorshipIDFAPESP: 03/11086-1
dc.description.sponsorshipIDCNPq: 490214/2005-3
dc.description.sponsorshipIDCNPq: 472193/2004-0
dc.format.extent41-48
dc.identifierhttp://dx.doi.org/10.1016/j.canlet.2008.12.022
dc.identifier.citationCancer Letters. Clare: Elsevier B.V., v. 278, n. 1, p. 41-48, 2009.
dc.identifier.doi10.1016/j.canlet.2008.12.022
dc.identifier.issn0304-3835
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31603
dc.identifier.wosWOS:000265712800007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofCancer Letters
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectMultiple myelomaen
dc.subjectSAGEen
dc.subjectGene expression profilingen
dc.subjectMeta-analysisen
dc.titleSAGE analysis highlights the importance of p53csv, ddx5, mapkapk2 and ranbp2 to multiple myeloma tumorigenesisen
dc.typeinfo:eu-repo/semantics/article
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