Iron restriction increases myoglobin gene and protein expression in Soleus muscle of rats

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dc.citation.issue4
dc.citation.volume88
dc.contributor.authorSouza, Janaina Sena de [UNIFESP]
dc.contributor.authorBrunetto, Erika L.
dc.contributor.authorNunes, Maria Tereza
dc.coverageRio Janeiro
dc.date.accessioned2020-07-31T12:47:37Z
dc.date.available2020-07-31T12:47:37Z
dc.date.issued2016
dc.description.abstractIron is an important trace element for proper cell functioning. It is present in cytochromes, hemoglobin and myoglobin (Mb), where it binds to oxygen. It is also an electron acceptor in the respiratory chain. Mb is an 18 kDa heme-protein, highly expressed in skeletal muscle and heart. The expression of several genes involved in the metabolism of iron is post-transcriptionally regulated by this element. Iron was shown to interfere with the polyadenylation step, modifying their poly (A) tail length and, as a consequence, their stability and translation rate. The aim of this study was to investigate whether iron supplementation or long and short-term restriction affects Mb gene and protein expression, as well as Mb mRNA poly(A) tail length, in cardiac and skeletal muscles of rats. Long-term iron restriction caused an increase in Mb gene and protein expression in Soleus muscle. No changes were observed in extensor digitorum longus muscle and heart. Short-term iron supplementation after iron deprivation did not alter Mb gene expression and mRNA poly(A) tail length in all tissues studied. These results indicate that Mb gene and protein expression is upregulated in response to iron deprivation, an effect that is tissue-specific and seems to occur at transcriptional level.en
dc.description.affiliationUniv Sao Paulo, ICB, Dept Fisiol & Biofis, Lab 129,Ave Prof Lineu Prestes 1524, BR-05508900 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Med, Lab Endocrinol Mol & Translac,Div Endocrinol, Rua Pedro de Toledo 669,11 Andar, BR-04039032 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista de Medicina, Departamento de Medicina, Laboratório Endocrinologia Molecular e Translacional, Divisão de Endocrinologia, Rua Pedro de Toledo, 669, 11º andar, Vila Clementino, 04039-032 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 07/6096-5
dc.description.sponsorshipIDCNPq: 305659/2009-0
dc.format.extent2277-2290
dc.identifierhttp://dx.doi.org/10.1590/0001-3765201620160173
dc.identifier.citationAnais Da Academia Brasileira De Ciencias. Rio Janeiro, v. 88, n. 4, p. 2277-2290, 2016.
dc.identifier.doi10.1590/0001-3765201620160173
dc.identifier.fileS0001-37652016000602277.pdf
dc.identifier.issn0001-3765
dc.identifier.scieloS0001-37652016000602277
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56945
dc.identifier.wosWOS:000391581800020
dc.language.isoeng
dc.publisherAcad Brasileira De Ciencias
dc.relation.ispartofAnais Da Academia Brasileira De Ciencias
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcardiac muscleen
dc.subjectironen
dc.subjectMyoglobinen
dc.subjectskeletal muscleen
dc.titleIron restriction increases myoglobin gene and protein expression in Soleus muscle of ratsen
dc.typeinfo:eu-repo/semantics/article
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