Crotamine toxicity and efficacy in mouse models of melanoma

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dc.contributor.authorPereira, Alexandre
dc.contributor.authorKerkis, Alexandre
dc.contributor.authorHayashi, Mirian A. F. [UNIFESP]
dc.contributor.authorPereira, Aparecida S. P.
dc.contributor.authorSilva, Fernando S.
dc.contributor.authorOliveira, Eduardo B.
dc.contributor.authorPrieto da Silva, Alvaro R. B.
dc.contributor.authorYamane, Tetsuo
dc.contributor.authorRadis-Baptista, Gandhi
dc.contributor.authorKerkis, Irina
dc.contributor.institutionButantan Inst
dc.contributor.institutionVet Act Ltda Genet Aplicada
dc.contributor.institutionVirol Lab
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Estado Amazonas
dc.contributor.institutionUniv Fed Ceara
dc.date.accessioned2016-01-24T14:17:12Z
dc.date.available2016-01-24T14:17:12Z
dc.date.issued2011-09-01
dc.description.abstractObjectives: Selective anticancer cell activity for both cell-penetrating and cationic antimicrobial peptides has previously been reported. As crotamine possesses activities similar to both of these, this study investigates crotamine's anticancer toxicity in vitro and in vivo.Research design and methods: in vitro cancer cell viability was evaluated after treatment with 1 and 5 mu g/ml of crotamine. in vivo crotamine cytotoxic effects in C57Bl/6J mice bearing B16-F10 primary cutaneous melanoma were tested, with two groups each containing 35 mice. the crotamine-treated group received 1 mu g/day of crotamine per animal, subcutaneously which was well tolerated; the untreated group received a placebo.Results: Crotamine at 5 mu g/ml was lethal to B16-F10, Mia PaCa-2 and SK-Mel-28 cells and inoffensive to normal cells. in vivo crotamine treatment over 21 days significantly delayed tumor implantation, inhibited tumor growth and prolonged the lifespan of the mice. Mice in the crotamine-treated group survived at significantly higher rates (n = 30/35) than those in the untreated group (n = 7/35) (significance calculated with the Kaplan-Meier estimator). the average tumor weight in the untreated group was 4.60 g but was only about 0.27 g in the crotamine-treated mice, if detectable.Conclusions: These data warrant further exploration of crotamine as a tumor inhibition compound.en
dc.description.affiliationButantan Inst, Ctr Appl Toxinol CAT CEPID, BR-05503900 São Paulo, Brazil
dc.description.affiliationButantan Inst, Genet Lab, BR-05503900 São Paulo, Brazil
dc.description.affiliationVet Act Ltda Genet Aplicada, Celltrovet, São Paulo, Brazil
dc.description.affiliationVirol Lab, São Paulo, Brazil
dc.description.affiliationFed Univ São Paulo UNIFESP, Dept Pharmacol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Ribeirao Preto Med Sch, BR-14049 Ribeirao Preto, Brazil
dc.description.affiliationUniv Estado Amazonas, Natl Inst Sci & Technol Environm Energy & Biodive, Manaus, Amazonas, Brazil
dc.description.affiliationUniv Fed Ceara, Inst Marine Sci, Fortaleza, Ceara, Brazil
dc.description.affiliationUnifespFed Univ São Paulo UNIFESP, Dept Pharmacol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent1189-1200
dc.identifierhttp://dx.doi.org/10.1517/13543784.2011.602064
dc.identifier.citationExpert Opinion On Investigational Drugs. London: Informa Healthcare, v. 20, n. 9, p. 1189-1200, 2011.
dc.identifier.doi10.1517/13543784.2011.602064
dc.identifier.issn1354-3784
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34038
dc.identifier.wosWOS:000293897900002
dc.language.isoeng
dc.publisherInforma Healthcare
dc.relation.ispartofExpert Opinion On Investigational Drugs
dc.rightsAcesso restrito
dc.rights.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dc.subjectantimicrobial peptidesen
dc.subjectcancer cells selective toxicityen
dc.subjectcell-penetrating peptidesen
dc.subjectchemotherapeutical compounden
dc.subjecttumor growth arresten
dc.titleCrotamine toxicity and efficacy in mouse models of melanomaen
dc.typeArtigo
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